ARID1A Antibody (JJ09-01)

Immunocytochemistry/Immunofluorescence: ARID1A Antibody (JJ09-01) [NBP2-67886] - Staining ARID1A in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ARID1A Antibody (JJ09-01) [NBP2-67886] - Analysis of paraffin-embedded rat testis tissue using anti-ARID1A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/Immunofluorescence: ARID1A Antibody (JJ09-01) [NBP2-67886] - Staining ARID1A in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: ARID1A Antibody (JJ09-01) [NBP2-67886] - Staining ARID1A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ARID1A Antibody (JJ09-01) [NBP2-67886] - Analysis of paraffin-embedded human spleen tissue using anti-ARID1A antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ARID1A Antibody (JJ09-01) [NBP2-67886] - Analysis of paraffin-embedded mouse brain tissue using anti-ARID1A antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ARID1A Antibody (JJ09-01) [NBP2-67886] - Analysis of paraffin-embedded mouse colon tissue using anti-ARID1A antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ARID1A Antibody (JJ09-01) [NBP2-67886] - Analysis of paraffin-embedded mouse testis tissue using anti-ARID1A antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ARID1A Antibody (JJ09-01) [NBP2-67886] - Analysis of paraffin-embedded human spleen tissue using anti-ARID1A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: ARID1A Antibody (JJ09-01) [NBP2-67886] - Analysis of paraffin-embedded mouse kidney tissue using anti-ARID1A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

ARID1A Antibody (JJ09-01) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human ARID1A aa 1,184-1,334 / 2,285. (SwissProt: O14497 Human; SwissProt: A2BH40 Mouse; Unigene: 61077 Rat)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ARID1A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:400

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ARID1A Antibody (JJ09-01)

• ARID domain-containing protein 1A
• AT rich interactive domain 1A (SWI- like)
• AT rich interactive domain 1A (SWI-like)
• AT-rich interactive domain-containing protein 1A
• B120SWI-like protein
• BAF250a
• BAF250SWI/SNF complex protein p270
• BM029
• brain protein 120
• BRG1-associated factor 250
• BRG1-associated factor 250a
• C10rf4
• C1orf4SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatinsubfamily F member 1
• chromatin remodeling factor p250
• hELD
• hOSA1
• matrix associated, actin dependent regulator of chromatin
• Osa homolog 1
• OSA1 nuclear protein
• OSA1
• P270
• SMARCF1
• subfamily f, member 1

Background

ARID1A encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain ARID1A genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARID1A