ARHGEF6 Antibody (2E5) - Azide and BSA Free

Western Blot: ARHGEF6 Antibody (2E5) [H00009459-M02] - Analysis of ARHGEF6 expression in transfected 293T cell line by ARHGEF6 monoclonal antibody (M02), clone 2E5.Lane 1: ARHGEF6 transfected lysate (Predicted MW: 87.5 KDa).Lane 2: Non-transfected lysate.

Proximity Ligation Assay: ARHGEF6 Antibody (2E5) [H00009459-M02] - Analysis of protein-protein interactions between CDC42 and ARHGEF6. HeLa cells were stained with anti-CDC42 rabbit purified polyclonal 1:1200 and anti-ARHGEF6 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Immunoprecipitation: ARHGEF6 Antibody (2E5) [H00009459-M02] - Analysis of ARHGEF6 transfected lysate using anti-ARHGEF6 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ARHGEF6 monoclonal antibody.

Sandwich ELISA: ARHGEF6 Antibody (2E5) [H00009459-M02] - Detection limit for recombinant GST tagged ARHGEF6 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Func, ICC/IF, IP
• Clone: 2E5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ARHGEF6 Antibody (2E5) - Azide and BSA Free Summary

• Immunogen: ARHGEF6 (AAH39856.1, 61 a.a. ~ 175 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QTEADCINNINDFLKGCATLQVEIFDPDDLYSGVNFSKVLSTLLAVNKATEDQLSERPCGRSSSLSAANTSQTNPQGAVSSTVSGLQRQSKTVEMTENGSHQLIVKARFNFKQTN
• Specificity: ARHGEF6 - Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6 (2E5)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARHGEF6
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Proximity Ligation Assay
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ARHGEF6 Antibody (2E5) - Azide and BSA Free

• alphaPIX
• Alpha-Pix
• COOL-2
• COOL2alpha
• KIAA0006Cool2
• mental retardation, X-linked 46
• MRX46
• PAK-interacting exchange factor alpha
• PIXA
• Rac/Cdc42 guanine exchange factor (GEF) 6
• Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6
• Rac/Cdc42 guanine nucleotide exchange factor 6
• rho guanine nucleotide exchange factor 6

Background

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It may form a complex with G proteins and stimulate Rho-dependent signals. This protein is activated by PI3-kinase. Mutations in this gene can cause X-chromosomal non-specific mental retardation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARHGEF6 Antibody (H00009459-M02)(1)

Publication using H00009459-M02

• Mazaki Y, Onodera Y, Higashi T et al. ARF1 recruits RAC1 to leading edge in neutrophil chemotaxis. Cell Commun Signal 2017-10-02 [PMID: 28969640]

Bioinformatics

• Gene Symbol: ARHGEF6
• Entrez:
  • 9459(Human)
• Uniprot:
  • AAH39856(Human)