ARHGAP1 Antibody [Alexa Fluor® 750]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 750

ARHGAP1 Antibody [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human ARHGAP1
  aa 1-62
  Accession # Q07960
• Specificity: Detects human, mouse, and rat ARHGAP1 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ARHGAP1 Antibody [Alexa Fluor® 750]

• ARHGAP1
• CDC42 GTPase-activating protein
• CDC42GAP
• CDC42GAPp50-RhoGAP
• GTPase-activating protein rhoOGAP
• p50rhoGAP
• Rho GTPase activating protein 1
• rho GTPase-activating protein 1
• RhoGAP
• RHOGAP1Cdc42GAP
• Rho-related small GTPase protein activator
• Rho-type GTPase-activating protein 1

Background

ARHGAP1 (also p50-RhoGAP and CDC42 GTPase-activating protein) is a 50 kDa member of the ARHGAP family of proteins. It is preferentially expressed in epithelium, and serves as a cytoplasmic protein that deactivates Rho-family GTPases. It does so by contributing an Arg residue (Arg282) to Cdc42 and RhoA, which activates their intrinsic GTPase activity and converts GTP-associated molecules into inactivating GDP molecules. Human ARHGAP1 is 439 amino acids (aa) in length and contains one CRAL-TRIO domain that binds small lipophilic molecules (aa 53-218), an SH3 binding site (aa 228-238) and one C-terminal RhoGAP domain (aa 244-431). There are phosphorylation sites at Ser51 and Tyr65. There is one potential splice form with a 24 aa substitution for aa 1-150 coupled to a 26 aa substitution for aa 300-439. Over aa 1-62, human ARHGAP1 shares 96% aa identity with mouse ARHGAP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q07960(Human)