Arginyl tRNA synthetase Antibody

Western Blot: Arginyl tRNA synthetase Antibody [NBP1-46148] - NBP1-46148 (1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Arginyl tRNA synthetase Antibody Summary

• Description: Novus Biologicals Goat Arginyl tRNA synthetase Antibody (NBP1-46148) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-Arginyl tRNA synthetase Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-SLQAERNKPTKN corresponding to internal region according to NP_002878.2.
• Epitope: internal region
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RARS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 75 kDa band observed in lysates of HeLa and HepG2 (calculated MW of 75.4 kDa band according to NP_002878.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Arginyl tRNA synthetase Antibody

• arginine tRNA ligase 1, cytoplasmic
• Arginine--tRNA ligase
• arginyl-tRNA synthetase
• arginyl-tRNA synthetase, cytoplasmic
• ArgRS
• DALRD1
• EC 6.1.1.19
• MGC8641

Background

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Arginyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Arginyl tRNA synthetase Antibody (NBP1-46148)(2)

Publications using NBP1-46148

• Theodoridis PR, Bokros M, Marijan D et al. Local translation in nuclear condensate amyloid bodies Proceedings of the National Academy of Sciences of the United States of America 2021-02-16 [PMID: 33568529] (ICC, Human)
• Bottoni A, Vignali C, Piccin D et al. Proteasomes and RARS modulate AIMP1/EMAP II secretion in human cancer cell lines. J Cell Physiol 2007-08-01 [PMID: 17443684]

Bioinformatics

• Gene Symbol: RARS1
• Entrez:
  • 5917(Human)
  • 104458(Mouse)
  • 287191(Rat)
• Uniprot:
  • NP_002878.2(Human)