ARFGAP1 Antibody - BSA Free

Western Blot: ARFGAP1 Antibody [NBP2-38787] - Analysis using Anti-ARFGAP1 antibody NBP2-38787 (A) shows similar pattern to independent antibody NBP2-14305 (B).

Immunocytochemistry/Immunofluorescence: ARFGAP1 Antibody [NBP2-38787] - Staining of human cell line CACO-2 shows localization to cytosol, the Golgi apparatus & vesicles. Antibody stainign is shown in green.

Immunohistochemistry-Paraffin: ARFGAP1 Antibody [NBP2-38787] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.

Immunohistochemistry-Paraffin: ARFGAP1 Antibody [NBP2-38787] - Staining of human pancreas shows strong cytoplasm granular positivity in exocrine glandular cells.

Immunohistochemistry-Paraffin: ARFGAP1 Antibody [NBP2-38787] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: ARFGAP1 Antibody [NBP2-38787] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ARFGAP1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: TLPSMVHRVSGQPQSVTASSDKAFEDWLNDDLGSYQGAQGNRYVGFGNTPPPQKKEDDFLNNAMSSLYSGWSSFTTGASRFASAAKEGATKFGSQASQKASEL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARFGAP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  ARFGAP1 Protein (NBP2-38787PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (86%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ARFGAP1 Antibody - BSA Free

• ADP-ribosylation factor 1 GTPase activating protein
• ADP-ribosylation factor 1 GTPase-activating protein
• ADP-ribosylation factor GTPase activating protein 1
• ADP-ribosylation factor GTPase-activating protein 1
• ARF GAP 1
• ARF1 GAP
• ARF1-directed GTPase-activating protein
• ARF1GAP
• ARFGAP1
• bA261N11.3
• FLJ10767
• GAP protein
• HRIHFB2281
• MGC39924

Background

ARFGAP1 is encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARFGAP1
• Uniprot:
  • Q8N6T3(Human)