ARF5 Antibody

Images

 
Western Blot: ARF5 Antibody [NBP1-31005] - Sample(30 ug of whole cell lysate)A:MOLT4 B:Raji 15% SDS PAGE, antibody diluted at 1:1500.
Immunocytochemistry: ARF5 Antibody [NBP1-31005] - Analysis of methanol-fixed HeLa, using ARF5 antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: ARF5 Antibody [NBP1-31005] - Paraffin-embedded human breast carcinoma. ARF5 antibody dilution: 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ARF5 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ARF5. The exact sequence is proprietary.
Localization
Golgi apparatus
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Sheep (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARF5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 40% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ARF5 Antibody

  • ADP-ribosylation factor 5

Background

ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-505
Species: Hu, Mu, Rt, Bv, Ca
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-41263
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IF
NBP2-26147
Species: Hu, Mu
Applications: WB, PEP-ELISA
NBP1-31004
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-02300
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NBP1-32606
Species: Mu
Applications: IHC, IHC-P
NBP1-77933
Species: Hu, Mu, Rt, Bv, Ca, Ch, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-22513
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-24614
Species: Hu, Mu, Rt, Op, Pm
Applications: WB
NBP1-88840
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
AF2420
Species: Hu
Applications: WB, IHC
AF6260
Species: Hu
Applications: WB
NBP1-52556
Species: Hu, Mu, Po, Bt, Bv, Ca, Mk, Pm, Rb
Applications: IHC-P
NBP1-85727
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-81480
Species: Hu
Applications: ICC/IF, IHC, IHC-P
H00011247-T01
Species: Hu
NBP2-23721
Species: Hu, Pm
Applications: WB, IHC, IHC-P
NBP1-31005
Species: Hu, Mu, Rt, Po, Bv, Sh
Applications: WB, ICC/IF, IHC, IHC-P

Publications for ARF5 Antibody (NBP1-31005) (0)

There are no publications for ARF5 Antibody (NBP1-31005).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ARF5 Antibody (NBP1-31005) (0)

There are no reviews for ARF5 Antibody (NBP1-31005). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ARF5 Antibody (NBP1-31005). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for antibodies against human ARF4 and ARF5 and I need to be able to distinguish between the two proteins. Do you have antibodies specific for each protein that won't cross react with the other protein?
    • I checked the sequence similarity between ARF4 and ARF5, and there is very high homology between the two proteins. We have not specifically tested our antibodies for cross-reactivity between the two, but I think it's likely that they will.

Secondary Antibodies

 

Isotype Controls

Additional ARF5 Products

Bioinformatics Tool for ARF5 Antibody (NBP1-31005)

Discover related pathways, diseases and genes to ARF5 Antibody (NBP1-31005). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ARF5 Antibody (NBP1-31005)

Discover more about diseases related to ARF5 Antibody (NBP1-31005).
 

Pathways for ARF5 Antibody (NBP1-31005)

View related products by pathway.

PTMs for ARF5 Antibody (NBP1-31005)

Learn more about PTMs related to ARF5 Antibody (NBP1-31005).
 

Research Areas for ARF5 Antibody (NBP1-31005)

Find related products by research area.

Blogs on ARF5

There are no specific blogs for ARF5, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ARF5 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol ARF5
Entrez
Uniprot