ARF4L Antibody

Western Blot: ARF4L Antibody [NBP1-51927] - (0.5ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ARF4L Antibody Summary

• Description: Novus Biologicals Goat ARF4L Antibody (NBP1-51927) is a polyclonal antibody validated for use in WB and ELISA. Anti-ARF4L Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KQDQPGALSAAEVEKR corresponding to internal region according to NP_001652.2.
• Specificity: This antibody is expected not to cross-react to other ARL4 products.
• Predicted Species: Mouse (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARL4D
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 22 kDa band observed in human lung lysates (calculated MW of 22.2 kDa band according to NP_001652.2).
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ARF4L Antibody

• ADP-ribosylation factor 4-like
• ADP-ribosylation factor-like 4D
• ADP-ribosylation factor-like protein 4D
• ADP-ribosylation factor-like protein 4L
• ARF4LADP-ribosylation factor-like 6
• ARL6

Background

ADP-ribosylation factor 4D is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL4D is closely similar to ARL4A and ARL4C and each has a nuclear localization signal and an unusually high guanine nucleotide exchange rate. This protein may play a role in membrane-associated intracellular trafficking. Mutations in this gene have been associated with Bardet-Biedl syndrome (BBS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARF4L Antibody (NBP1-51927)(1)

Publication using NBP1-51927

• Nonaka Y, Tsuda N, Shichijo S et al. Recognition of ADP-ribosylation factor 4-like by HLA-A2-restricted and tumor-reactive cytotoxic T lymphocytes from patients with brain tumors. Tissue Antigens 2002-10-01 [PMID: 12472661]

Bioinformatics

• Gene Symbol: ARL4D
• Entrez:
  • 379(Human)
  • 80981(Mouse)
• Uniprot:
  • P49703(Human)