ARF4 Antibody

Images

 
Western Blot: ARF4 Antibody [NBP2-26147] - NIH3T3 (A) and HeLa (B) lysates (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ARF4 Antibody [NBP2-26147] - Human Thymus tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ARF4 Antibody Summary

Immunogen
Peptide with sequence C-SEMTDKLGLQSLRN corresponding to internal region according to NP_001651.1.
Predicted Species
Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ARF4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.3 - 1 ug/mL
Application Notes
WB: Approx. 19 kDa band observed in NIH3T3 and HeLa lysates (calculated MW of 20.5 kDa band according to NP_001651.1).
Publications
Read Publication using NBP2-26147.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ARF4 Antibody

  • ADP-ribosylation factor 4
  • ARF2ADP-ribosylation factor 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ARF4 Antibody (NBP2-26147)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for ARF4 Antibody (NBP2-26147). (Showing 1 - 1 of 1 FAQ).

  1. I was looking for antibodies against human ARF4 and ARF5 and I need to be able to distinguish between the two proteins. Do you have antibodies specific for each that don't cross react with the other protein?
    • The sequence similarity between ARF4 and ARF5 shows very high homology between the two proteins. We have not specifically tested for cross-reactivity between the antibodies, but I think it's very likely that they will cross react.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ARF4
Entrez