ARF4 Antibody

Western Blot: ARF4 Antibody [NBP2-26147] - NIH3T3 (A) and HeLa (B) lysates (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: ARF4 Antibody [NBP2-26147] - Human Thymus tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ARF4 Antibody Summary

• Immunogen: Peptide with sequence C-SEMTDKLGLQSLRN corresponding to internal region according to NP_001651.1.
• Predicted Species: Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARF4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: WB: Approx. 19 kDa band observed in NIH3T3 and HeLa lysates (calculated MW of 20.5 kDa band according to NP_001651.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ARF4 Antibody

• ADP-ribosylation factor 4
• ARF2ADP-ribosylation factor 2

Background

The ARF4 gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins thatstimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and asactivators of phospholipase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARF4 Antibody (NBP2-26147)(1)

Publication using NBP2-26147

• Jang SY, Jang SW, Ko J. Regulation of ADP-ribosylation factor 4 expression by small leucine zipper protein and involvement in breast cancer cell migration. Cancer Lett 2012-01-28 [PMID: 22004728]

FAQs for ARF4 Antibody (NBP2-26147). (Showing 1 - 1 of 1 FAQ).

1. I was looking for antibodies against human ARF4 and ARF5 and I need to be able to distinguish between the two proteins. Do you have antibodies specific for each that don't cross react with the other protein?
   • The sequence similarity between ARF4 and ARF5 shows very high homology between the two proteins. We have not specifically tested for cross-reactivity between the antibodies, but I think it's very likely that they will cross react.

Bioinformatics

• Gene Symbol: ARF4
• Entrez:
  • 378(Human)
  • 11843(Mouse)
  • 79120(Rat)