APPD Antibody

Western Blot: APPD Antibody [NBP1-90124] - Analysis in control (vector only transfected HEK293T lysate) and PLEKHF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry-Paraffin: APPD Antibody [NBP1-90124] - Staining of human skeletal muscle shows distinct positivity in myocytes.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

APPD Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: MVDHLANTEINSQRIAAVESCFGASGQPLALPGRVLLGEGVLTKECRKKAKPRIFFLFNDILVYGSIVLNKRKYRSQHIIPLEEVTLELLPETLQAKNRWMIKTAKKSFVVSAASATERQEWISHIEE
• Predicted Species: Mouse (98%), Rat (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PLEKHF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  APPD Protein (NBP1-90124PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for APPD Antibody

• Apoptosis-inducing protein
• APPDapoptosis-inducing protein D
• LAPF
• Lysosome-associated apoptosis-inducing protein containing PH and FYVE domains
• MGC4090
• PH and FYVE domain-containing protein 1
• PH domain-containing family F member 1
• PHAFIN1
• phafin-1
• pleckstrin homology domain containing, family F (with FYVE domain) member 1
• pleckstrin homology domain-containing family F member 1
• ZFYVE15phafin 1
• Zinc finger FYVE domain-containing protein 15

Background

APPD is composed of the pleckstrin homology domain and FYVE domain. It may induce apoptosis and is known to have interaction with TNNT1 and KIAA1279.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLEKHF1
• Entrez:
  • 79156(Human)
• Uniprot:
  • Q96S99(Human)