Apolipoprotein M/ApoM Antibody

Immunohistochemistry-Paraffin: Apolipoprotein M/ApoM Antibody [NB100-57088] - (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Apolipoprotein M/ApoM Antibody Summary

• Immunogen: Peptide with sequence PRNQEACELSNN corresponding to C-Terminus according to NP_061974.2.
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: APOM
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:8000
• Application Notes: WB: Preliminary experiments gave an approx. 50 kDa band in human kidney and liver lysates after 0.2 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 21.3 kDa band according to NP_061974.2. We do note that ApoM is a glycoprotein which makes a higher size band to be expected. The 50 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human liver shows strong stain of cytoplasm in select hepatocytes.
• Control:

  Apolipoprotein M/ApoM Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Apolipoprotein M/ApoM Antibody

• Apolipoprotein M
• APOM
• apo-M
• G3A
• HSPC336
• MGC22400
• NG20
• NG20-like protein
• Protein G3a

Background

The protein encoded by this gene is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Two transcript variants encoding two different isoforms have been found for this gene, but only one of them has been fully characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Apolipoprotein M/ApoM Antibody (NB100-57088)(2)

Publications using NB100-57088

• Mosialou I, Zannis VI, Kardassis D. Regulation of human apolipoprotein m gene expression by orphan and ligand-dependent nuclear receptors. J Biol Chem 2010-10-01 [PMID: 20660599] (Human)
• Xu N, Nilsson-Ehle P, Ahren B. Correlation of apolipoprotein M with leptin and cholesterol in normal and obese subjects. J Nutr Biochem 2004-10-01 [PMID: 15542348]

Bioinformatics

• Gene Symbol: APOM
• Entrez:
  • 55937(Human)
• Uniprot:
  • O95445()