Apolipoprotein F Antibody

Images

 
Western Blot: Apolipoprotein F Antibody [NBP1-20882] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Apolipoprotein F Antibody [NBP1-20882] - Staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Apolipoprotein F Antibody Summary

Immunogen
Peptide with sequence C-KSYDLDPGAGSLE corresponding to C-Terminus according to NP_001629.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
APOF
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Immunohistochemistry 2.5 - 3.8 ug/ml
  • Immunohistochemistry-Paraffin 2.5 - 3.8 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 35 kDa band observed in human liver lysates (calculated MW of 35.4 kDa band according to NP_001629.1). IHC-P: Human liver shows staniing of droplets in the cytoplasm of hepatocytes.
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-20882.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Apolipoprotein F Antibody

  • Apo-F
  • apolipoprotein F
  • DKFZp781G18150
  • Lipid transfer inhibitor protein
  • LTIP
  • MGC22520

Background

The product of this gene is one of the minor apolipoproteins found in plasma. This protein forms complexes with lipoproteins and may be involved in transport and/or esterification of cholesterol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Apolipoprotein F Antibody (NBP1-20882)(1)

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol APOF
Entrez
Uniprot