Apolipoprotein F Antibody

Western Blot: Apolipoprotein F Antibody [NBP1-20882] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Apolipoprotein F Antibody [NBP1-20882] - Staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Apolipoprotein F Antibody Summary

• Immunogen: Peptide with sequence C-KSYDLDPGAGSLE corresponding to C-Terminus according to NP_001629.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: APOF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2.5 - 3.8 ug/ml
  • Immunohistochemistry-Paraffin 2.5 - 3.8 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 35 kDa band observed in human liver lysates (calculated MW of 35.4 kDa band according to NP_001629.1). IHC-P: Human liver shows staniing of droplets in the cytoplasm of hepatocytes.
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Apolipoprotein F Antibody

• Apo-F
• apolipoprotein F
• DKFZp781G18150
• Lipid transfer inhibitor protein
• LTIP
• MGC22520

Background

The product of this gene is one of the minor apolipoproteins found in plasma. This protein forms complexes with lipoproteins and may be involved in transport and/or esterification of cholesterol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Apolipoprotein F Antibody (NBP1-20882)(1)

Publication using NBP1-20882

• Wang X, Driscoll DM, Morton RE. Molecular cloning and expression of lipid transfer inhibitor protein reveals its identity with apolipoprotein F. J Biol Chem 1999-01-15 [PMID: 9880564]

Bioinformatics

• Gene Symbol: APOF
• Entrez:
  • 319(Human)
• Uniprot:
  • Q13790(Human)