Human Apolipoprotein E/ApoE ELISA Kit (Colorimetric)

ELISA: Human Apolipoprotein E/ApoE ELISA Kit (Colorimetric) [NBP2-82117] - Samples were spiked with high concentrations of Human Apolipoprotein E/ApoE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Apolipoprotein E/ApoE ELISA Kit (Colorimetric) [NBP2-82117] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell supernatant
• Standard Curve Range: 23.44 - 1500 ng/mL
• Sensitivity: 14.06 ng/mL

Human Apolipoprotein E/ApoE ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human Apolipoprotein E/ApoE in samples. No significant cross-reactivity or interference between Human Apolipoprotein E/ApoE and analogues was observed.
• Standard Curve Range: 23.44 - 1500 ng/mL
• Sensitivity: 14.06 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 6%
• Intra-Assay: CV% < 6%
• Spike Recovery: average = 101% for serum; average = 102% for EDTA plasma; average = 97% for cell culture media
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: APOE

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Apolipoprotein E/ApoE ELISA Kit (Colorimetric)

• APOE
• Apo-E
• Apolipoprotein E
• apolipoprotein E3
• late onset)
• MGC1571

Background

Apolipoprotein E (APOE) is an apolipoprotein located in chylomicrons and intermediate-density lipoproteins that is necessary for the normal catabolism of triglyceride-rich lipoprotein constituents in the liver. APOE has recently been shown to be involseveral biological processes other than lipoprotein transport, including cognition and immunoregulation.

Lipoproteins are responsible for carrying cholesterol and other fats through the bloodstream as little packages and are essential for the normal breakdown of these molecules. In particular, apolipoprotein E is a major component of specific lipoproteins called very low-density lipoproteins (VLDL). A major function of VLDLs is to remove excess cholesterol from the blood and carry it to the liver for processing. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes.

The three Apo E isoforms are strongly associated with cardiovascular disease and Alzheimer's disease, so ApoE antibodies have become useful tools for research in both of these areas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: APOE