Human Apolipoprotein E/ApoE ELISA Kit (Colorimetric)

ELISA: Apolipoprotein E/ApoE ELISA Kit [HRP] [KA1031] - Standard curve

• Reactivity: Hu
• Applications: ELISA, NULL
• Suitable Sample Type: Cell Culture Supernatants, CSF, Plasma, Serum
• Standard Curve Range: 0.016 to 1 ug/mL
• Sensitivity: 4.3 ng/mL

Human Apolipoprotein E/ApoE ELISA Kit (Colorimetric) Summary

• Description: APOE (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human APOE.
• Standard Curve Range: 0.016 to 1 ug/mL
• Sensitivity: 4.3 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 7.60%
• Intra-Assay: CV% < 4.60%
• Spike Recovery: 99.50%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: APOE

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Apolipoprotein E/ApoE ELISA Kit (Colorimetric)

• APOE
• Apo-E
• Apolipoprotein E
• apolipoprotein E3
• late onset)
• MGC1571

Background

Apolipoprotein E (APOE) is an apolipoprotein located in chylomicrons and intermediate-density lipoproteins that is necessary for the normal catabolism of triglyceride-rich lipoprotein constituents in the liver. APOE has recently been shown to be involseveral biological processes other than lipoprotein transport, including cognition and immunoregulation.

Lipoproteins are responsible for carrying cholesterol and other fats through the bloodstream as little packages and are essential for the normal breakdown of these molecules. In particular, apolipoprotein E is a major component of specific lipoproteins called very low-density lipoproteins (VLDL). A major function of VLDLs is to remove excess cholesterol from the blood and carry it to the liver for processing. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes.

The three Apo E isoforms are strongly associated with cardiovascular disease and Alzheimer's disease, so ApoE antibodies have become useful tools for research in both of these areas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Apolipoprotein E/ApoE ELISA Kit (KA1031)(11)

Publications using KA1031

• Chandra B, Shiny N, Glenn B et al. Neuroinflammation in neuronopathic Gaucher disease: Role of microglia and NK cells, biomarkers, and response to substrate reduction therapy. Elife. 2022-08-16 [PMID: 35972072]
• Furuya H, Pagano I, Chee K et al. Comparison of Commercial ELISA Kits, a Prototype Multiplex Electrochemoluminescent Assay, and a Multiplex Bead-Based Immunoassay for Detecting a Urine-Based Bladder-Cancer-Associated Diagnostic Signature. Diagnostics (Basel). 2019-10-29 [PMID: 31671775]
• Kara E, Marks JD, Roe AD et al. A flow cytometry-based in vitro assay reveals that formation of apolipoprotein E (ApoE)-amyloid beta complexes depends on ApoE isoform- and cell type. J Biol Chem 2018-06-27 [PMID: 29950521]
• Shimizu Y, Furuya H, Bryant Greenwood P et al. A multiplex immunoassay for the non-invasive detection of bladder cancer. J Transl Med 2016-01-30 [PMID: 26830497] (ELISA)
• Chen LM, Chang M, Dai Y et al. External validation of a multiplex urinary protein panel for the detection of bladder cancer in a multicenter cohort. Cancer Epidemiol Biomarkers Prev. 2014-09-01 [PMID: 24920641] (ELISA, Human)
• Rosser CJ, Chang M, Dai Y et al. Urinary Protein Biomarker Panel for the Detection of Recurrent Bladder Cancer. Cancer Epidemiol Biomarkers Prev. 2014-04-08 [PMID: 24714076]
• Rosser CJ, Ross S, Chang M et al. Multiplex protein signature for the detection of bladder cancer in voided urine samples. J Urol. 2013-12-01 [PMID: 23764080] (Human)
• Goodison S, Chang M, Dai Y et al. A multi-analyte assay for the non-invasive detection of bladder cancer. PLoS One. 2012-01-01 [PMID: 23094052]
• Urquidi V, Goodison S, Ross S et al. Diagnostic potential of urinary alpha1-antitrypsin and apolipoprotein E in the detection of bladder cancer. J Urol. 2012-12-01 [PMID: 23088986] (Human)
• Burt TD, Agan BK, Marconi VC et al. Apolipoprotein (apo) E4 enhances HIV-1 cell entry in vitro, and the APOE epsilon4/epsilon4 genotype accelerates HIV disease progression. Proc Natl Acad Sci U S A. 2008-06-24 [PMID: 18562290]
• Schultz MR, Lyons MJ, Franz CE et al. Apolipoprotein E genotype and memory in the sixth decade of life. Neurology. 2008-05-06 [PMID: 18235080]

FAQs for Apolipoprotein E/ApoE ELISA Kit (KA1031). (Showing 1 - 2 of 2 FAQs).

1. Does APOE (Human) ELISA Kit KA1031 work in saliva?
   •  We do believe this kit APOE (Human) ELISA Kit KA1031  will work in saliva.   We would recommend a titration for determination of the optimal dilutions since we have not made the determination.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: APOE