Apolipoprotein E/ApoE Antibody (GT27711) - Azide and BSA Free

ELISA: Apolipoprotein E/ApoE Antibody (GT27711) [NBP3-13692] - Indirect ELISA analysis performed by coating plate with recombinant full-length human apolipoprotein E (400-6.25 ng/mL). Coated protein was probed with Apolipoprotein E/ApoE antibody [GT27711] (NBP3-13692) (1 ug/mL). Mouse IgG antibody (HRP) (NBP2-19382) (1:10000) was used to detect bound primary antibody.

Sandwich ELISA: Apolipoprotein E/ApoE Antibody (GT27711) [NBP3-13692] - Sandwich ELISA detection of recombinant full-length human apolipoprotein E using Apolipoprotein E/ApoE antibody [GT27711] (NBP3-13692) as capture antibody at concentration of 5 ug/mL and Apolipoprotein E/ApoE antibody [C2C3], C-term as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.

Western Blot: Apolipoprotein E/ApoE Antibody (GT27711) [NBP3-13692] - Human plasma (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E/ApoE antibody [GT27711] (NBP3-13692) diluted at 1:25000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Western Blot: Apolipoprotein E/ApoE Antibody (GT27711) [NBP3-13692] - Rat plasma (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E/ApoE antibody [GT27711] (NBP3-13692) diluted at 1:25000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

• Reactivity: Hu
• Applications: ELISA
• Clone: GT27711
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Apolipoprotein E/ApoE Antibody (GT27711) - Azide and BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Apolipoprotein E/ApoE. The exact sequence is proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: APOE
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Apolipoprotein E/ApoE Antibody (GT27711) - Azide and BSA Free

• APOE
• Apo-E
• Apolipoprotein E
• apolipoprotein E3
• late onset)
• MGC1571

Background

Apolipoprotein E (APOE) is an apolipoprotein located in chylomicrons and intermediate-density lipoproteins that is necessary for the normal catabolism of triglyceride-rich lipoprotein constituents in the liver. APOE has recently been shown to be involseveral biological processes other than lipoprotein transport, including cognition and immunoregulation.

Lipoproteins are responsible for carrying cholesterol and other fats through the bloodstream as little packages and are essential for the normal breakdown of these molecules. In particular, apolipoprotein E is a major component of specific lipoproteins called very low-density lipoproteins (VLDL). A major function of VLDLs is to remove excess cholesterol from the blood and carry it to the liver for processing. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes.

The three Apo E isoforms are strongly associated with cardiovascular disease and Alzheimer's disease, so ApoE antibodies have become useful tools for research in both of these areas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APOE