Non-human Primate Apolipoprotein B/ApoB ELISA Kit Summary
| Standard Curve Range |
7.8 - 500 ng/mL |
| Sensitivity |
7 ng/mL |
| Inter-Assay |
%CV < 10.8 |
| Intra-Assay |
%CV < 3.2 |
| Spike Recovery |
90 - 114% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit |
| Gene |
APOB |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Apo ELISA buffer concentrate (5x)
- Detection mAb LDL 11, biotin (1 mg/ml)
- Human apoB ELISA standard: Purified LDL in glycerol
- Pre-coated ELISA strip plate: Anti-apoB mAbs LDL 20/17
- Sample solvent
- Stop solution
- Streptavidin-HRP diluent
- Streptavidin-HRP
- TM B substrate
- Wash buffer concentrate (20x)
|
Alternate Names for Non-human Primate Apolipoprotein B/ApoB ELISA Kit
Background
Apolipoprotein B (apo B) in human plasma is a major protein of low density lipoproteins (LDL). The molecular mass is 260 kDa. Apo B binds to specific receptors on cell membranes and is involved in removal of LDL and very low density lipoprotein (VLDL) cholesterol from circulation.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Apolipoprotein B/ApoB ELISA Kit (NBP3-11737). (Showing 1 - 1 of 1 FAQ).
-
What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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