Human Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric)

ELISA: Apolipoprotein A-I/ApoA1 ELISA Kit [Biotin] [KA0460] - Standard curve

Product Discontinued

Human Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric) Summary

• Description: APOA1 (Human) ELISA Kit is a competitive enzyme immunoassay for the quantitative measurement of human APOA1 in plasma, serum.
• Standard Curve Range: 1.25 to 40 ug/mL
• Sensitivity: 0.13 ug/mL
• Assay Type: Competitive ELISA
• Inter-Assay: CV% < 7.30%
• Intra-Assay: CV% < 4.60%
• Spike Recovery: 96.00%
• Sample Volume: 25 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: APOA1

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification
• Application Notes: Useful for quantification.

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric)

• Alp-1
• APOA1
• Apo-AI
• apoA-I
• Apolipoprotein A1
• Apolipoprotein AI
• Apolipoprotein A-I
• Brp-14
• Ltw-1
• Lvtw-1
• MGC117399
• Sep-1
• Sep-2

Background

Apolipoprotein A1 (apo A1) is the major polypeptide of the human plasma high density lipoprotein (HDL). The structure and function of the apo A1 gene are of interest because of the inverse correlation shown between HDL levels and coronary heart disease (1). The 267 amino acid precursor initially undergoes intracellular co-translational proteolytic cleavage into proapoA-I. ProapoA-I is secreted from the cell and was isolated from thoracic duct lymph in the apoA-I1 isoform position. Results indicate that apo A1 is present in human plasma, and undergoes post-translational proteolytic cleavage to mature plasma apoA-I (2). Cleavage of this protein with cyanogen bromide yields four fragments designated in the order of elution from Bio-Gel P-30 as CNBr I, II, III, and IV (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Apolipoprotein A-I/ApoA1 ELISA Kit (KA0460)(7)

Publications using KA0460

• Montanola A, de Retana SF, Lopez-Rueda A et al. ApoA1, ApoJ and ApoE Plasma Levels and Genotype Frequencies in Cerebral Amyloid Angiopathy. Neuromolecular Med 2016-03-01 [PMID: 26661731]
• Castle JW, Kent KP, Fan Y et al. Therapeutic ultrasound: Increased HDL-Cholesterol following infusions of acoustic microspheres and apolipoprotein A-I plasmids. Atherosclerosis 2015-07-01 [PMID: 25969892] (ICC/IF)
• Chiang SL, Ou TT, Wu YJ et al. Increased level of MSU crystal-bound protein apolipoprotein A-I in acute gouty arthritis. Scand J Rheumatol. 2014-09-02 [PMID: 25178483]
• De Bock M, Beguin Y, Leprince P et al. Comprehensive plasma profiling for the characterization of graft-versus-host disease biomarkers. Talanta. 2014-03-18 [PMID: 24840443]
• Davidson WS, Thompson TB., et al. The structure of apolipoprotein A-I in high density lipoproteins. J Biol Chem 2007-05-25 [PMID: 17526499]
• Shao B, Oda MN, Vaisar T et al. Pathways for oxidation of high-density lipoprotein in human cardiovascular disease. Curr Opin Mol Ther 2006-06-01 [PMID: 16774039]
• Forte TM, Nichols AV, Gong EL et al. Electron microscopic study on reassembly of plasma high density apoprotein with various lipids. Biochim Biophys Acta. 1971-11-05 [PMID: 5167106]

FAQs for Apolipoprotein A-I/ApoA1 ELISA Kit (KA0460). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: APOA1