Apolipoprotein A-I/ApoA1 Antibody [HRP]

Product Discontinued

Apolipoprotein A-I/ApoA1 Antibody [HRP] Summary

• Immunogen: Purified human apolipoprotein A1
• Specificity: This product reacts with human apolipoprotein A1 and does not react with other human plasma proteins. In ELISA the following reactivities are observed: Human apolipoprotein A1 100% Human apolipoprotein B <3% Human albumin <1% Apolipoprotein A1 is a single polypeptide with a molecular weight of 28 kDa. It is the major protein component of high density lipoprotein particles and functions in preventing the accumulation of cholesterol loaded macrophages which deposit on the arterial wall as foam cells.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: APOA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:20000
  • Western Blot 1:100-1:2000
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555
• Control:

  Apolipoprotein A-I/ApoA1 Overexpression Lysate

Reactivity Notes

Human. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.5% BSA
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for Apolipoprotein A-I/ApoA1 Antibody [HRP]

• Alp-1
• APOA1
• Apo-AI
• ApoA-I
• Apolipoprotein A1
• Apolipoprotein AI
• Apolipoprotein A-I
• Brp-14
• Ltw-1
• Lvtw-1
• MGC117399
• Sep-1
• Sep-2

Background

Apolipoprotein A I promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids; an 18 amino acid signal peptide is removed co-translationally and a 6 amino acid propeptide is cleaved post-translationally. Apolipoprotein A I is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease. The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I. The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

&#9888; WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Review for Apolipoprotein A-I/ApoA1 Antibody (NB100-66391) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer ELISA Human 11/30/2015

Summary

• Application: ELISA
• Sample Tested: Human Plasma
• Species: Human

Bioinformatics

• Gene Symbol: APOA1