APLP-1 Antibody (354020)

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 354020
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

APLP-1 Antibody (354020) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human APLP-1
  Gln34-Glu580
  Accession # P51693
• Specificity: Detects human and mouse APLP-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human APLP-2 or recombinant mouse APLP-2 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: APLP1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for APLP-1 Antibody (354020)

• amyloid beta (A4) precursor-like protein 1
• amyloid precursor-like protein 1
• amyloid-like protein 1
• APLP1
• APLP-1
• APLPAPLP-1

Background

APLP-1 is a transmembrane metalloprotein that is expressed in central neurons. Similar to APP and APLP-2, APLP-1 is susceptible to cleavage by various secretases, generating multiple fragments from the extracellular and intracellular domains. These include peptides similar to the amyloidogenic A beta peptides and a cytoplasmic fragment that associates with Fe65 family proteins and functions as a transcriptional activator. The extracellular domain contains heparin and collagen binding regions and is 89% identical between human and mouse.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for APLP-1 Antibody (MAB3908)(3)

Publications using MAB3908

• Simoes S, Neufeld J. L, et al. Tau and other proteins found in Alzheimer's disease spinal fluid are linked to retromer-mediated endosomal traffic in mice and humans. Sci Transl Med 11/25/2020 [PMID: 33239387]
• Chera L. Maarouf, Tyler A. Kokjohn, Charisse M. Whiteside, MiMi P. Macias, Walter M. Kalback, Marwan N. Sabbagh et al. Molecular Differences and Similarities between Alzheimer's Disease and the 5XFAD Transgenic Mouse Model of Amyloidosis Biochemistry Insights 2013-11-21 [PMID: 25210460]
• Maarouf C, Kokjohn T, Walker D, Whiteside C, Kalback W, Whetzel A, Sue L, Serrano G, Jacobson S, Sabbagh M, Reiman E, Beach T, Roher A Biochemical assessment of precuneus and posterior cingulate gyrus in the context of brain aging and Alzheimer's disease. PLoS ONE, 2014-08-28;9(8):e105784. 2014-08-28 [PMID: 25166759]

Bioinformatics

• Gene Symbol: APLP1
• Uniprot:
  • P51693()