Human APJ/Apelin receptor ELISA Kit (Colorimetric)

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Tissue homogenates and other biological fluids
• Standard Curve Range: 15.625 - 1000 pg/mL (example only; lot dependent)
• Sensitivity: 5.7 pg/mL (example only; lot dependent)

Human APJ/Apelin receptor ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 4.5 hours
• Standard Curve Range: 15.625 - 1000 pg/mL (example only; lot dependent)
• Sensitivity: 5.7 pg/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: APLNR

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human APJ/Apelin receptor ELISA Kit (Colorimetric)

• AGTRL1
• AGTRL1HG11
• angiotensin II receptor-like 1
• Angiotensin receptor-like 1
• apelin receptor
• APJ (apelin) receptor
• APJ receptor
• APJ
• APJFLJ96609
• APJR
• APLNR
• FLJ90771
• G protein-coupled receptor APJ
• G-protein coupled receptor APJ
• G-protein coupled receptor HG11
• HG11 orphan receptor
• HG11
• MGC45246

Background

APJ or Apelin Receptor, is an angiotensin-1-like receptor that is expressed abundantly in the corpus callosum and spinal cord. The human apelin receptor has been localized to chromosome 11. In porcine spinal cord, APJ mRNA was detected at a higher level in white matter than in gray matter (Matsumoto et al., 1996). APJ binds apelin, which induces cell acidification (Tatemoto et al., 1998). APJ expression has been reported in blood, brain, spinal cord, and vessel. ESTs have been isolated from brain, head/neck, lung, and placenta libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for APJ/Apelin receptor ELISA Kit (NBP3-54360). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: APLNR