APJ/Apelin receptor Antibody (72133R) [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu
• Applications: Flow, IHC
• Clone: 72133R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

APJ/Apelin receptor Antibody (72133R) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human APJ
  Met1-Asp380
  Accession # P35414
• Specificity: Detects human APJ in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for APJ/Apelin receptor Antibody (72133R) [CoraFluor™ 1]

• AGTRL1
• AGTRL1HG11
• angiotensin II receptor-like 1
• Angiotensin receptor-like 1
• apelin receptor
• APJ (apelin) receptor
• APJ receptor
• APJ
• APJFLJ96609
• APJR
• APLNR
• FLJ90771
• G protein-coupled receptor APJ
• G-protein coupled receptor APJ
• G-protein coupled receptor HG11
• HG11 orphan receptor
• HG11
• MGC45246

Background

APJ or Apelin Receptor, is an angiotensin-1-like receptor that is expressed abundantly in the corpus callosum and spinal cord. The human apelin receptor has been localized to chromosome 11. In porcine spinal cord, APJ mRNA was detected at a higher level in white matter than in gray matter (Matsumoto et al., 1996). APJ binds apelin, which induces cell acidification (Tatemoto et al., 1998). APJ expression has been reported in blood, brain, spinal cord, and vessel. ESTs have been isolated from brain, head/neck, lung, and placenta libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.