APCDD1 Antibody (2584B) [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: Flow
• Clone: 2584B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 405

APCDD1 Antibody (2584B) [Alexa Fluor® 405] Summary

• Immunogen: Mouse myeloma cell line, NS0-derived human APCDD1
  Leu27-His492
  Accession # Q8J025
• Specificity: Detects human APCDD1 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for APCDD1 Antibody (2584B) [Alexa Fluor® 405]

• Adenomatosis polyposis coli down-regulated 1 protein
• adenomatosis polyposis coli down-regulated 1
• APC Down Regulated 1
• APCDD1
• B7323
• DRAPC1
• HYPT1
• protein APCDD1

Background

APCDD1 (Adenomatosis Polyposis Coli Down-Regulated 1) is a membrane bound glycoprotein that is an endogenous inhibitor of the Wnt signaling pathway (1, 2). Inhibition of Wnt signaling by APCDD1 plays a role in adipocyte differentiation as well as pathogenesis of disease (2-4). Mature human APCDD1 consists of a 465 amino acid (aa) extracellular domain (ECD), a 19 aa transmembrane domain, and a 1 aa cytoplasmic region. Human APCDD1 shares a 95% and 94% amino acid sequence similarity with rat and mouse respectively. APCDD1 interacts in vitro with Wnt-3a and LRP5 (5). It is expressed in a broad repertoire of cell types which might regulate a diversity of biological processes controlled by Wnt signaling, including breast cancer cell invasion (6), osteogenic differentiation of human dental follicle cells (7), vascular remodeling and barrier maturation of retinal blood vessels (4) and hair follicle miniaturization (5). Extracellular domain of APCDD1 has been shown to co-immunoprecipitate with recombinant Wnt-3a and LRP5 (1), suggesting that APCDD1 can modulate the Wnt pathway by potential interactions with Wnt-3a and LRP5 at the cell surface.

1. Mazzoni, J. et al. (2017) Neuron. 96:1055.
2. Yiew, N.K.H. et al. (2017) J. Biol. Chem. 292:6312.
3. Shimomura, Y. (2010) Nature 464:1043.
4. Kandimalla, R. (2017) Oncogenesis 6:e308.
5. Yutaka Shimomura, et al. (2010) Nature 464:1043.
6. Sung-Gook CHO, (2017) Oncology Letters 14:4845.
7. Viale-Bouroncle S. et al. (2015) Biochem Biophys Res Commun 457(3):314.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q8J025()