APCDD1 Antibody (2584B) [Alexa Fluor™ Plus 647]

• Reactivity: Hu
• Clone: 2584B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 647

APCDD1 Antibody (2584B) [Alexa Fluor™ Plus 647] Summary

• Specificity: Detects human APCDD1 in direct ELISAs.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for APCDD1 Antibody (2584B) [Alexa Fluor™ Plus 647]

• Adenomatosis polyposis coli down-regulated 1 protein
• adenomatosis polyposis coli down-regulated 1
• APC Down Regulated 1
• APCDD1
• B7323
• DRAPC1
• HYPT1
• protein APCDD1

Background

APCDD1 (Adenomatosis Polyposis Coli Down-Regulated 1) is a membrane bound glycoprotein that is an endogenous inhibitor of the Wnt signaling pathway (1, 2). Inhibition of Wnt signaling by APCDD1 plays a role in adipocyte differentiation as well as pathogenesis of disease (2-4). Mature human APCDD1 consists of a 465 amino acid (aa) extracellular domain (ECD), a 19 aa transmembrane domain, and a 1 aa cytoplasmic region. Human APCDD1 shares a 95% and 94% amino acid sequence similarity with rat and mouse respectively. APCDD1 interacts in vitro with Wnt-3a and LRP5 (5). It is expressed in a broad repertoire of cell types which might regulate a diversity of biological processes controlled by Wnt signaling, including breast cancer cell invasion (6), osteogenic differentiation of human dental follicle cells (7), vascular remodeling and barrier maturation of retinal blood vessels (4) and hair follicle miniaturization (5). Extracellular domain of APCDD1 has been shown to co-immunoprecipitate with recombinant Wnt-3a and LRP5 (1), suggesting that APCDD1 can modulate the Wnt pathway by potential interactions with Wnt-3a and LRP5 at the cell surface.

1. Mazzoni, J. et al. (2017) Neuron. 96:1055.
2. Yiew, N.K.H. et al. (2017) J. Biol. Chem. 292:6312.
3. Shimomura, Y. (2010) Nature 464:1043.
4. Kandimalla, R. (2017) Oncogenesis 6:e308.
5. Yutaka Shimomura, et al. (2010) Nature 464:1043.
6. Sung-Gook CHO, (2017) Oncology Letters 14:4845.
7. Viale-Bouroncle S. et al. (2015) Biochem Biophys Res Commun 457(3):314.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.