AP3D1 Antibody

Western Blot: AP3D1 Antibody [NBP2-27158] - analysis using AP3 Delta antibody. HeLa lysate in the 1) absence and 2) presence of immunizing peptide, probed with AP3 Delta antibody at 2 ug/ml. goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: AP3D1 Antibody [NBP2-27158] - IHC of human rectum tissue probed with AP3 Delta antibody at 5 ug/ml.

• Reactivity: Hu, Pm
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

AP3D1 Antibody Summary

• Description: Novus Biologicals Rabbit AP3D1 Antibody (NBP2-27158) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 530-580 of human AP3 Delta was used as the immunogen for the antibody.
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AP3D1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5ug/ml
  • Western Blot 2-5ug/ml~

Reactivity Notes

The AP3 amino acid sequence used as immunogen for this antibody is 100% homologous in human, chimpanzee, orangutan, new world monkey, rhesus monkey, and 63% homologous in panda, carolina anole, opossum, and 58% homologous in taipan, zebra finch, horse, dog, pig and 53% homologous in cow, rat, mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for AP3D1 Antibody

• Adapter-related protein complex 3 subunit delta-1
• adaptin, delta
• adaptor-related protein complex 3, delta 1 subunit
• ADTD
• AP-3 complex delta subunit, partial CDS
• AP-3 complex subunit delta
• AP-3 complex subunit delta-1
• delta adaptin
• Delta-adaptin
• delta-adaptin, partial CDS
• hBLVR
• subunit of putative vesicle coat adaptor complex AP-3

Background

AP3 delta (AP3D1) is part of the AP3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. Adaptor protein complex 3 (AP3) is a heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta- type subunit AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and a small adaptin (sigma-type subunit APS1 or AP3S2). The AP3 Delta subunit is implicated in intracellular biogenesis and trafficking of pigment granules and possibly platelet dense granules and neurotransmitter vesicles. Five isoforms of the human protein are produced by alternative splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AP3D1