AP-2 gamma Antibody

Immunohistochemistry-Paraffin: AP-2 gamma Antibody [NB100-1516] - Staining of AP-2 gamma in paraffin embedded Human Skin using NB100-1516 at 2.5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: AP-2 gamma Antibody [NB100-1516] - (4ug/ml) Staining of Human Skin. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

AP-2 gamma Antibody Summary

• Immunogen: Peptide with sequence C-NKTLEKMEKHRK corresponding to C-Terminus according to NP_003213.1.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TFAP2C
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 - 3 ug/ml
  • Immunohistochemistry-Paraffin 2 - 3 ug/ml
  • Peptide ELISA Detection limit 1:128000
• Application Notes: WB: WB experiments gave bands at approx. 75 kDa band and 22 kDa band in human placenta and ovary lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 49.2 kDa band according to NP_003213.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). IHC-P: Human skin shows nuclear staining of keratinocytes.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AP-2 gamma Antibody

• Activating enhancer-binding protein 2 gamma
• AP2 gamma
• AP-2 gamma
• AP2-GAMMA
• ERF1
• estrogen receptor factor 1
• hAP-2g
• TFAP2C
• TFAP2G
• transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma)
• transcription factor AP-2 gamma (activating enhancer-binding protein 2 gamma)
• transcription factor AP-2 gamma
• Transcription factor ERF-1

Background

The protein encoded by this gene is a sequence-specific DNA-binding transcription factor involved in the activation of several developmental genes. The encoded protein can act as either a homodimer or heterodimer with other family members and is induced during retinoic acid-mediated differentiation. It plays a role in the development of the eyes, face, body wall, limbs, and neural tube.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AP-2 gamma Antibody (NB100-1516)(1)

Publication using NB100-1516

• Hoei-Hansen CE, Rajpert-De Meyts E, Carlsen E et al. Fast-track Article: A subfertile patient diagnosed with testicular carcinoma in situ by immunocytological staining for AP-2{gamma} in semen samples: Case report. Hum Reprod 2005-01-13 [PMID: 15650041]

Bioinformatics

• Gene Symbol: TFAP2C
• Entrez:
  • 7022(Human)
  • 21420(Mouse)
  • 362280(Rat)
• Uniprot:
  • Q92754()