GSPT2 Antibody (OTI2G9) - Azide and BSA Free

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Western Blot: GSPT2 Antibody (OTI2G9) - Azide and BSA Free [NBP2-71768] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GSPT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: GSPT2 Antibody (OTI2G9) - Azide and BSA Free [NBP2-71768] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GSPT2.
Western Blot: GSPT2 Antibody (OTI2G9) - Azide and BSA Free [NBP2-71768] - Analysis of extracts (10ug) from 2 different cell lines by using anti-GSPT2 monoclonal antibody.(1:200)
Immunocytochemistry/ Immunofluorescence: GSPT2 Antibody (OTI2G9) - Azide and BSA Free [NBP2-71768] - Staining of HeLa cells using anti-GSPT2 mouse monoclonal antibody.
CyTOF-ready: GSPT2 Antibody (OTI2G9) - Azide and BSA Free [NBP2-71768] - Analysis of Jurkat cells, using anti-GSPT2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
CyTOF-ready: GSPT2 Antibody (OTI2G9) - Azide and BSA Free [NBP2-71768] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-GSPT2 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, CyTOF-ready
Clone
OTI2G9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

GSPT2 Antibody (OTI2G9) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human GSPT2 (NP_060564) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GSPT2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Western Blot 1:2000
Theoretical MW
68.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for GSPT2 Antibody (OTI2G9) - Azide and BSA Free

  • eRF3b
  • ERF3BGST2
  • eukaryotic peptide chain release factor GTP-binding subunit ERF3B
  • Eukaryotic peptide chain release factor subunit 3b
  • FLJ10441
  • G1 to S phase transition 2
  • G1 to S phase transition protein 2 homolog

Background

GSPT2 is closely related to GSPT1 (MIM 139259), a GTP-binding protein that plays an essential role at the G1- to S-phase transition of the cell cycle in yeast and human cells. GSPT1 is a positive regulator of translational accuracy and, in a binary complex with eRF1 (MIM 600285), functions as a polypeptide chain release factor.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GSPT2