AP-2 gamma Antibody (AP2g 6E4/4)

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IP
Clone
AP2g 6E4/4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

AP-2 gamma Antibody (AP2g 6E4/4) Summary

Immunogen
Bacterially produced AP-2a/AP-2g fusion protein (c-terminal half of AP-2 gamma)
Epitope
C terminus.
Localization
Nuclear
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TFAP2C
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
Application Notes
Antigen recovery will improve staining on paraffin sections.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Notes

Positive control(s): MCF7 cells.

Alternate Names for AP-2 gamma Antibody (AP2g 6E4/4)

  • Activating enhancer-binding protein 2 gamma
  • AP2 gamma
  • AP-2 gamma
  • AP2-GAMMA
  • ERF1
  • estrogen receptor factor 1
  • hAP-2g
  • TFAP2C
  • TFAP2G
  • transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma)
  • transcription factor AP-2 gamma (activating enhancer-binding protein 2 gamma)
  • transcription factor AP-2 gamma
  • Transcription factor ERF-1

Background

AP2 gamma is a sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limbs and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TFAP2C
Entrez