Human Antidiuretic Hormone ELISA Kit (Colorimetric)

ELISA: Human Antidiuretic Hormone ELISA Kit (Colorimetric) [NBP2-62140] - A typical standard curve for Antidiuretic Hormone ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Human serum, human plasma, tissue culture media
• Standard Curve Range: 4.10 - 1,000 pg/ml
• Sensitivity: 2.84 pg/ml

Human Antidiuretic Hormone ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 4.10 - 1,000 pg/ml
• Sensitivity: 2.84 pg/ml
• Assay Type: Competitive ELISA
• Inter-Assay: Low Concentration %CV - 9.5% Medium Concentration %CV - 6.4% High Concentration %CV - 8.6%
• Intra-Assay: Low Concentration %CV - 14.3% Medium Concentration %CV - 6.7% High Concentration %CV - 6.0%
• Spike Recovery: Tissue Culture Media - 116.2%
  Tissue Culture Media + 10% Bovine Serum - 100.0%
  Assay Buffer 28 - 111.4%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: AVP

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Antidiuretic Hormone ELISA Kit (Colorimetric)

• ADH
• arginine vasopressin
• ARVP
• Vasopressin

Background

This gene encodes a member of the vasopressin/oxytocin family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the neuropeptide hormone arginine vasopressin, and two other peptides, neurophysin 2 and copeptin. Arginine vasopressin is a posterior pituitary hormone that is synthesized in the supraoptic nucleus and paraventricular nucleus of the hypothalamus. Along with its carrier protein, neurophysin 2, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis where it is either stored or secreted into the bloodstream. The precursor is thought to be activated while it is being transported along the axon to the posterior pituitary. Arginine vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney, and also causes vasoconstriction of the peripheral vessels. This hormone can contract smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in this gene cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI). This gene is present in a gene cluster with the related gene oxytocin on chromosome 20. [provided by RefSeq, Nov 2015]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Antidiuretic Hormone ELISA Kit (NBP2-62140). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: AVP