Annexin V Apoptosis Detection Kit

Product Discontinued

Annexin V Apoptosis Detection Kit Summary

• Description: Simple one-step assay to detect apoptotic, necrotic and live cells. Apoptotic cells show red fluorescence, dead cells show green fluorescence and live cells show little or no fluorescence. No need to fix the cells.
  
  Detection method: Fluorescence microscopy or Flow cytometry.Applications: Detecting apoptosis in living cells by Flow cytometry or Fluorescence microscopy.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab273274)
• Kit Type: Apoptosis Detection Kit
• Gene: ANXA5

Applications/Dilutions

• Application Notes: Flow cytometry: Use FL1 channel for SYTOX Green dye (Ex/Em = 488/530 nm) and FL2 channel for Annexin V-PE (Ex/Em = 488/578 nm).

Packaging, Storage & Formulations

• Storage: Store at 4C.

Alternate Names for Annexin V Apoptosis Detection Kit

• Anchorin CII
• annexin A5
• Annexin V
• annexin-5
• ANX5
• ANXA5
• ANXV
• Calphobindin I
• Endonexin II
• ENX2CBP-I
• Lipocortin V
• PAP-I
• Placental anticoagulant protein 4
• Placental anticoagulant protein I
• PP4
• Thromboplastin inhibitor
• VAC-alpha
• Vascular anticoagulant-alpha

Background

The Annexin V-PE Apoptosis Detection Kit Plus is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily be detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need of fixation. The Annexin V-PE Apoptosis Detection Kit Plus includes annexin V-PE, SYTOX green dye, and binding buffer. The SYTOX green dye is impermeable to live cells and apoptotic cells, but stains necrotic cells with intense green fluorescence by binding to cellular nucleic acids. After staining a cell population with annexin V-PE and SYTOX Green dye in the provided binding buffer, apoptotic cells show red fluorescence, dead cells show green fluorescence and live cells show little or no fluorescence. These populations can easily be distinguished by Fluorescence microscopy using FITC and PE filters or by flow cytometry using the FL1 channel (Ex. 488 nm/Em. 530 nm) for SYTOX Green dye and FL2 channel for Annexin V-PE (Ex. 488 nm/Em. 578 nm).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ANXA5