Annexin V Apoptosis Detection Kit

Product Discontinued

Annexin V Apoptosis Detection Kit Summary

• Description: Simple one-step procedure. The assay can be directly performed on live cells, without the need of fixation. The kit includes annexin V-FITC, SYTOX green dye, and binding buffer. The SYTOX green dye is impermeant to live cells and apoptotic cells, but stains necrotic cells with intense green fluorescence by binding to cellular nucleic acids. Dead cells show a higher level of green fluorescence and live cells show little or no fluorescence. Detection method: Flow cytometry (Ex/Em = 488/530 nm).
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab273273)
• Kit Type: Apoptosis Detection Kit
• Gene: ANXA5

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Store at 4C.

Alternate Names for Annexin V Apoptosis Detection Kit

• Anchorin CII
• annexin A5
• Annexin V
• annexin-5
• ANX5
• ANXA5
• ANXV
• Calphobindin I
• Endonexin II
• ENX2CBP-I
• Lipocortin V
• PAP-I
• Placental anticoagulant protein 4
• Placental anticoagulant protein I
• PP4
• Thromboplastin inhibitor
• VAC-alpha
• Vascular anticoagulant-alpha

Background

The Annexin V Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily be detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. In addition

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ANXA5