Human Annexin V ELISA Kit (Colorimetric)

Product Discontinued

Human Annexin V ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 6.3 - 400 ng/mL
• Sensitivity: 1.2 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 3.80%
• Intra-Assay: CV% < 2.00%
• Spike Recovery: 107%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: ANXA5

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: The human anti-Annexin V ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of antibodies to human Annexin V (Vac- alpha)

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Annexin V ELISA Kit (Colorimetric)

• Anchorin CII
• annexin A5
• Annexin V
• annexin-5
• ANX5
• ANXA5
• ANXV
• Calphobindin I
• Endonexin II
• ENX2CBP-I
• Lipocortin V
• PAP-I
• Placental anticoagulant protein 4
• Placental anticoagulant protein I
• PP4
• Thromboplastin inhibitor
• VAC-alpha
• Vascular anticoagulant-alpha

Background

Annexins are a family of calcium-dependent phospholipid-binding proteins. All annexins have been shown to have putative binding site for protein kinases C but only annexin V would possess a potential pseudo-substrate site. Thus annexin V seems to modulate the activity of some PKCs on their substrates. Annexin V was found to play a major role in matrix vesicle-initiated cartilage calcification as a collage-regulated calcium channel. Annexin V?s preferential binding partner is phosphatidylserine (PS). PS is predominantly located in membrane leaflets, which face the cytosol. However, recent findings show that each cell type has the molecular machinery to expose PS at its cell surface. This machinery is activated during the execution of apoptosis. Once PS is exposed at the cell surface it exhibits procoagulant and proinflammatory activities. Annexin V will bind to the PS-exposing apoptotic cell and can inhibit the procoagulant and proinflammatory activities of the dying cell. These findings together with the presence of Annexin V in the extracellular space depict a novel pathophysiological significance for Annexin V in vivo. The occurrence of autoantibodies to annexin V has been described in several pathological disorders. It is concluded that extracellular annexin V provides an antigenic stimulus for autoantibody production. Such autoantibodies may have a detrimental role interfering with putative functions of annexin V.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Annexin V ELISA Kit (NBP1-91251). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: ANXA5