Annexin V Antibody (VAA-33) [FITC]

Product Discontinued

Annexin V Antibody (VAA-33) [FITC] Summary

• Immunogen: Recombinant Annexin V
• Specificity: NB100-64303 specifically recognizes human Annexin V, a phospholipid binding protein which binds with high affinity and selectivity to phosphatidylserine (PS), in the presence of calcium. The translocation of PS from the cytosolic leaflet to the outer leaflet of the plasma membrane, due to a loss in plasma membrane asymmetry during apoptosis, acts as an in vivo signal for the removal of the dying cell by phagocytosis. Detection of PS using Annexin V therefore provides a reliable diagnostic tool for the indication of early programmed cell death. Annexin V has also been identified as an anticoagulant protein in the blood coagulation cascade, by acting as an inhibitor of prothrombin activation. The presence of antibodies to Annexin V, are associated with systemic lupus erythematosus (SLE), recurrent spontaneous abortions and systemic sclerosis (SSc). Clone VAA-33 has been reported to work in western blotting applications.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANXA5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry Neat
• Application Notes: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
• Control:

  Annexin V Overexpression Lysate

Reactivity Notes

Reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris (pH 7.4), 100 mM NaCl, 1.0% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for Annexin V Antibody (VAA-33) [FITC]

• Anchorin CII
• annexin A5
• Annexin V
• annexin-5
• ANX5
• ANXA5
• ANXV
• Calphobindin I
• Endonexin II
• ENX2CBP-I
• Lipocortin V
• PAP-I
• Placental anticoagulant protein 4
• Placental anticoagulant protein I
• PP4
• Thromboplastin inhibitor
• VAC-alpha
• Vascular anticoagulant-alpha

Background

Annexin V is encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANXA5