Mouse Annexin A2 ELISA Kit (Chemiluminescence)

ELISA: Mouse Annexin A2 ELISA Kit (Chemiluminescence) [NBP2-68223] - Samples were spiked with high concentrations of Mouse Annexin A2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse Annexin A2 ELISA Kit (Chemiluminescence) [NBP2-68223] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.31-20 ng/mL
• Sensitivity: 0.19 ng/mL

Mouse Annexin A2 ELISA Kit (Chemiluminescence) Summary

• Specificity: This kit recognizes Mouse ANXA2 in samples. No significant cross-reactivity or interference between Mouse ANXA2 and analogues was observed.
• Standard Curve Range: 0.31-20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-CLIA
• Inter-Assay: CV% < 9.35%
• Intra-Assay: CV% < 8.42%
• Spike Recovery: 100-118%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Chemiluminescence)
• Gene: ANXA2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Annexin A2 ELISA Kit (Chemiluminescence)

• Annexin A2
• Annexin II
• annexin-2
• ANX2
• ANX2L4
• ANX2L4LPC2
• ANXA2
• CAL1H
• Calpactin I heavy chain
• calpactin I heavy polypeptide
• Calpactin-1 heavy chain
• chromobindin 8
• chromobindin-8
• LIP2
• LIP2PAP-IV
• Lipocortin II
• Lipocortin-2
• LPC2D
• LPC2DP36
• p36
• PAP-IV
• Placental anticoagulant protein IV
• Protein I

Background

The Annexins are a family of structurally similar proteins. Annexins bind to phospholipids and may be involved in regulation of membrane transport, membrane channel activity, and interaction of the cell membrane with the extracellular matrix. Annexin II is a calcium regulated, membrane binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Annexin A2 ELISA Kit (NBP2-68223)(1)

Publication using NBP2-68223

• Song Z, Xu Y, Zhang L et al. Comprehensive Proteomic Profiling of Urinary Exosomes and Identification of Potential Non-invasive Early Biomarkers of Alzheimer's Disease in 5XFAD Mouse Model Front Genet 2020-11-05 [PMID: 33250918] (ELISA, Mouse)

FAQs for Annexin A2 ELISA Kit (NBP2-68223). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ANXA2