Annexin A2 Antibody (JA42-30)

Western Blot: Annexin A2 Antibody (JA42-30) [NBP2-67155] - Analysis of Annexin A2 on different lysates using anti-Annexin A2 antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: Human kidney Lane 3: Murine testis

Immunocytochemistry/Immunofluorescence: Annexin A2 Antibody (JA42-30) [NBP2-67155] - Staining Annexin A2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Annexin A2 Antibody (JA42-30) [NBP2-67155] - Analysis of paraffin-embedded human prostate tissue using anti-Annexin A2 antibody. Counter stained with hematoxylin.

Flow Cytometry: Annexin A2 Antibody (JA42-30) [NBP2-67155] - Analysis of K562 cells with Annexin A2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Immunohistochemistry-Paraffin: Annexin A2 Antibody (JA42-30) [NBP2-67155] - Analysis of paraffin-embedded human colon cancer tissue using anti-Annexin A2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Annexin A2 Antibody (JA42-30) [NBP2-67155] - Analysis of paraffin-embedded human liver tissue using anti-Annexin A2 antibody. Counter stained with hematoxylin.

Product Discontinued

Annexin A2 Antibody (JA42-30) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Annexin A2 aa 26-75 / 339. (SwissProt: P07355 Human; SwissProt: P07356 Mouse; SwissProt: Q07936 Rat)
• Localization: Basement membrane. Melanosome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ANXA2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Annexin A2 Antibody (JA42-30)

• Annexin A2
• Annexin II
• annexin-2
• ANX2
• ANX2L4
• ANX2L4LPC2
• ANXA2
• CAL1H
• Calpactin I heavy chain
• calpactin I heavy polypeptide
• Calpactin-1 heavy chain
• chromobindin 8
• chromobindin-8
• LIP2
• LIP2PAP-IV
• Lipocortin II
• Lipocortin-2
• LPC2D
• LPC2DP36
• p36
• PAP-IV
• Placental anticoagulant protein IV
• Protein I

Background

The Annexins are a family of structurally similar proteins. Annexins bind to phospholipids and may be involved in regulation of membrane transport, membrane channel activity, and interaction of the cell membrane with the extracellular matrix. Annexin II is a calcium regulated, membrane binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANXA2