Annexin A2 Antibody (1G7) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02] - Localization of annexin A2 at the focal adhesions: KS1767 cells co-stained for paxillin (FITC-conjugated secondary antibody, green) and annexin A2 (Alexa 647-conjugated secondary antibody, red). An enlarged inset (white dotted line) is included for prompt visualization. Nuclei are visualized with DAPI (blue); scale bar, 10um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-017-03470-w), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02] - Co-localization analysis: KS1767 cells grown overnight onto circular coverslips were incubated for 2h at 37degrees C with biotinylated LGRFYAASG-pen, or unconjugated LGRFYAASG (10uM). Untreated cells were used as controls. IF staining of biotinylated peptides (avidin-FITC, green) and annexin A2 (Cy3-conjugated secondary antibody, red) was analyzed with Fiji ImageJ. Co-localized pixels are visualized in white (arrows), and Manders' overlap coefficients are reported in the graph (**P<0.01; ***P<0.001 versus LGRFYAASG-pen). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-017-03470-w), licensed under a CC-BY license.

Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02] - MDA-MB-231 subclones whole cell lysates were loaded with 30 ug/lane. 10% SDS-PAGE. ANXA2 Antibody (H00000302-M02) was used for primary antibody: 1:2000, 4C, overnight. WB image submitted by a verified customer review.

Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02] - ANXA2 monoclonal antibody (M02), clone 1G7 Analysis of ANXA2 expression in HepG2.

Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02] - Analysis of ANXA2 expression in transfected 293T cell line by ANXA2 monoclonal antibody (M02), clone 1G7.Lane 1: ANXA2 transfected lysate(40.4 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02] - Analysis of monoclonal antibody to ANXA2 on HeLa cell. Antibody concentration 10 ug/ml.

Immunocytochemistry/ Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02] - ANXA2 could regulate the activation of NF-kappa B through combined with the subunit p50. a, b. The change in localization of endogenous p50 was analyzed by IF-IC following treatment with or without TNF-alpha (100 ng/ml) for 1 h. Nuclei were stained with DAPI. The nuclear localization of endogenous p50 in each group were quantitated as a graph. The plotted error bars represent mean ± SEM. The nuclear localization of p50 was quantified according to data from 5 random figures in each group. c. Interaction between ANXA2 & p50 in SK-N-BE(2) cells was validated through Co-IP & analyzed by western blotting with the indicated antibodies **: P < 0.01. All results are from at least three independent experiments Image collected & cropped by CiteAb from the following publication (//jeccr.biomedcentral.com/articles/10.1186/s13046-017-0581-6), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, ICC/IF, IP
• Clone: 1G7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Annexin A2 Antibody (1G7) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ANXA2 (AAH66955, 19 a.a. ~ 357 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISVMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD
• Specificity: ANXA2 - annexin A2
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANXA2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28652618).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Annexin A2 Antibody (1G7) - Azide and BSA Free

• Annexin A2
• Annexin II
• annexin-2
• ANX2
• ANX2L4
• ANX2L4LPC2
• ANXA2
• CAL1H
• Calpactin I heavy chain
• calpactin I heavy polypeptide
• Calpactin-1 heavy chain
• chromobindin 8
• chromobindin-8
• LIP2
• LIP2PAP-IV
• Lipocortin II
• Lipocortin-2
• LPC2D
• LPC2DP36
• p36
• PAP-IV
• Placental anticoagulant protein IV
• Protein I

Background

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Annexin A2 Antibody (H00000302-M02)(7)

Publications using H00000302-M02

• Tramutola A, Abate G, Lanzillotta C et al. Protein nitration profile of CD3+ lymphocytes from Alzheimer disease patients: Novel hints on immunosenescence and biomarker detection. Free Radic Biol Med 2018-10-12 [PMID: 30321702]
• Wang Y, Chen K, Cai Y et al. Annexin A2 could enhance multidrug resistance by regulating NF-?B signaling pathway in pediatric neuroblastoma Journal of Experimental & Clinical Cancer Research 2017-12-01 [PMID: 28814318]
• Na Pombejra S, Salemi M, Phinney BS, Gelli A et al. The Metalloprotease, Mpr1, Engages AnnexinA2 to Promote the Transcytosis of Fungal Cells across the Blood-Brain Barrier. Front Cell Infect Microbiol 2017-06-30 [PMID: 28713781]
• Staquicini DI, Rangel R, Guzman-Rojas L et al. Intracellular targeting of annexin A2 inhibits tumor cell adhesion, migration, and in vivo grafting Sci Rep 2017-06-26 [PMID: 28652618] (ICC/IF, Mouse)
• Zakrzewicz D, Didiasova M, Zakrzewicz A et al. The interaction with caveolae-associated proteins regulates enolase-1 subcellular localization. Biochem J. 2014-06-01 [PMID: 24628430]
• Deng FY, Lei SF, Zhang Y et al. Peripheral blood monocyte-expressed ANXA2 geneis involved in pathogenesis of osteoporosis in humans. Mol Cell Proteomics. 2011-08-04 [PMID: 21817168]
• Menegay M, Lee D, Tabbara KF et al. Proteomic Analysis of Climatic Keratopathy Droplets. Invest Ophthalmol Vis Sci;49(7):2829-2837. 2008-01-01 [PMID: 18378572]

Review for Annexin A2 Antibody (H00000302-M02) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Yongkang Yang WB Human 09/12/2021

Summary

• Application: Western Blot
• Sample Tested: MDA-MB-231
• Species: Human
• Lot: H1121-1G7

Bioinformatics

• Gene Symbol: ANXA2
• Entrez:
  • 302(Human)
• OMIM:
  • 151740(Human)
• Uniprot:
  • AAH66955(Human)