Annexin A11 Antibody

Western Blot: Annexin A11 Antibody [NB300-875] - Western blot analysis of Annexin A11 in HeLa lysate using NB300-875 at 0.2 ug/ ml.

Immunohistochemistry-Paraffin: Annexin A11 Antibody [NB300-875] - Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Annexin A11 Antibody Summary

• Immunogen: Peptide with sequence YPGYPPPPGGYPP-C corresponding to N-Terminus according to NP_001148.1, NP_665875.1, NP_665876.1.
• Specificity: NP_001148.1; NP_665875.1 and NP_665876.1 are varients that encode the same protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ANXA11
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.2 - 1 ug/ml
• Application Notes: WB: Approx. 55 kDa band observed in HeLa lysate (predicted MW of approx. 58 kDa band according to NP_001148.1). IHC-P: Human placenta shows strong staining of Basement Membrane in trophoblasts.
• Control:

  Annexin A11 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Annexin A11 Antibody

• Annexin A11
• Annexin-11
• ANX1156-kD
• ANXA11
• CAP50
• CAP-50

Background

This protein is a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain the calcium-dependent phospholipid-binding sites. The encoded protein is a 56-kD antigen recognized by sera from patients with various autoimmune diseases. Transcript variants encoding the same isoform have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Annexin A11 Antibody (NB300-875)(1)

Publication using NB300-875

• Misaki Y, Pruijn GJ, van der Kemp AW, van Venrooij WJ. The 56K autoantigen is identical to human annexin XI. J Biol Chem 1994-02-11 [PMID: 7508441]

Bioinformatics

• Gene Symbol: ANXA11
• Entrez:
  • 311(Human)
• Uniprot:
  • NP_001148(Human)