Annexin A1 Antibody

Western Blot: Annexin A1 Antibody [H00000301-D01P] - Analysis of ANXA1 expression in mouse lung.

Immunocytochemistry/Immunofluorescence: Annexin A1 Antibody [H00000301-D01P] - Analysis of antibody to ANXA1 on HeLa cell. Antibody concentration 20 ug/ml.

Western Blot: Annexin A1 Antibody [H00000301-D01P] - Analysis of ANXA1 expression in Hela S3 NE.

Western Blot: Annexin A1 Antibody [H00000301-D01P] - Analysis of ANXA1 expression in Raw 264.7.

Western Blot: Annexin A1 Antibody [H00000301-D01P] - Analysis of ANXA1 expression in human pancreas.

Western Blot: Annexin A1 Antibody [H00000301-D01P] - Analysis of ANXA1 expression in human placenta.

Western Blot: Annexin A1 Antibody [H00000301-D01P] - Analysis of ANXA1 expression in transfected 293T cell line by ANXA1 polyclonal antibody.Lane 1: ANXA1 transfected lysate(38.70 KDa).Lane 2: Non-transfected lysate.

Product Discontinued

Annexin A1 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: ANXA1 (NP_000691.1, 1 a.a. - 346 a.a.) full-length human protein. MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN
• Specificity: Reacts with annexin A1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ANXA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Antibody reactivity against tissue and transfected lysate in WB and as a detection antibody in ELISA. IHC-P and FACS usages reported in scientific literature (PMID: 25827591).

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 25827591).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Annexin A1 Antibody

• Annexin A1
• annexin I (lipocortin I)
• Annexin I
• Annexin-1
• ANX1
• ANXA1
• Calpactin II
• calpactin-2
• chromobindin-9
• Lipocortin I
• LPC1
• LPC1annexin-1
• p35
• Phospholipase A2 inhibitory protein

Background

Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Annexin A1 Antibody (H00000301-D01P)(2)

Publications using H00000301-D01P

• Capik SF, White BJ, Larson RL et al. Effect of oral administration of meloxicam prior to transport on inflammatory mediators and leukoctye function of cattle at feedlot arrival Am. J. Vet. Res. 2017-12-01 [PMID: 29182392] (Bovine)
• Senthilkumaran C, Hewson J, Ollivett TL, Bienzle D. Localization of annexins A1 and A2 in the respiratory tract of healthy calves and those experimentally infected with Mannheimia haemolytica Veterinary Research. 2015-02-01 [PMID: 25827591] (ICC/IF, IHC-P, FLOW, Bovine)
  
  Details:
  Annexin A2 antibody used for IHC-P on tracheal, bronchial and lung tissues from 2-month-old healthy male Holstein calves which were experimentally infected or not with Mannheimia haemolytica. The IHC-P assay involved heat-induced epitope retrieval with pressure cooker/approximately 120C temperature using sodium citrate buffer pH 6, treatment of slides with Dako's endogenous enzyme blocker and serum-free protein blocker, incubation of slides with primary antibody at 1 ug/150 ul concentration for ON at 4C, detection with HRP conjugated secondary antibody and NovaRED chromogen followed by hematoxylin counterstain.

Bioinformatics

• Gene Symbol: ANXA1
• Entrez:
  • 301(Human)
• Uniprot:
  • NP_000691.1(Human)