Annexin A1 Antibody (ANEX 5E4/1)

Product Discontinued

Annexin A1 Antibody (ANEX 5E4/1) Summary

• Immunogen: Mixture of native membrane proteins from human bone tissues which would include whole native annexin proteins.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANXA1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: Positive control(s): Endothelial cells.
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Annexin A1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Annexin A1 Antibody (ANEX 5E4/1)

• Annexin A1
• annexin I (lipocortin I)
• Annexin I
• Annexin-1
• ANX1
• ANXA1
• Calpactin II
• calpactin-2
• chromobindin-9
• Lipocortin I
• LPC1
• LPC1annexin-1
• p35
• Phospholipase A2 inhibitory protein

Background

Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I has phospholipase A2 inhibitory activity, promotes membrane fusion and is involved in exocytosis. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. Annexin 1 was found to be one of the most highly upregulated genes in hairy cell leukemia. Immunostaining using antibody directed against Annexin A1 protein may prove useful in the diagnosis of hairy cell leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANXA1
• Entrez:
  • 301(Human)