Recombinant Human Angiostatin Protein

Product Discontinued

Recombinant Human Angiostatin Protein Summary

• Description: A recombinant protein corresponding to PLG.
  
  

  Source:E. coli

• Details of Functionality: The activity is assayed on anti-proliferation and anti-migration of endothelial cells in vitro and anti-angiogenesis in vivo. The specific activity of anti-migration of endothelial cells in vitro is 0.55x105 Units/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PLG
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Functional
  • SDS-Page
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: From 20 mM Sodium Acetate, pH 5.5, 4% mannitol
• Concentration: Lyoph
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute in sterile distilled H2O or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Store at 4C for 1 week or -20C for long-term storage.

Alternate Names for Recombinant Human Angiostatin Protein

• Angiostatin
• HAE4

Background

Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. A specific inhibitor of endothelial cell growth and angiogenesis. Displays increased inhibitory activity (ED50 =70 nM) relative to kringles 1-4 (ED50 = 135 nM). Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PLG