Angiopoietin-like Protein 2/ANGPTL2 Antibody [Unconjugated]

Western blot shows lysates of human heart tissue, human aorta tissue, and human uterus tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Angiopoietin‑like Protein 2/ANGPTL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2084) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Angiopoietin‑like Protein 2/ANGPTL2 at approximately 55-64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Product Discontinued

Angiopoietin-like Protein 2/ANGPTL2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-like 2
  Gln23-Phe492
  Accession # Q9UKU9
• Specificity: Detects human Angiopoietin-like 2 (ANGPTL2) in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse ANGPTL2 is observed and less than 1% cross-reactivity with recombinant human (rh) ANGPTL6 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ANGPTL2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-like Protein 2/ANGPTL2 Antibody [Unconjugated]

• angiopoietin-like 2
• Angiopoietin-like Protein 2
• angiopoietin-related protein 2
• ANGPTL2
• ANGRP2
• ARP2HARP
• MGC8889

Background

ANGPTL2, also known as angiopoietin-related protein 2 (ARP2), contains an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain, which are structurally conserved in angiopoietins. Unlike angiopoietins which are ligands for TIE2, ANGPTL2 is an orphan ligand that does not bind TIE2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Angiopoietin-like Protein 2/ANGPTL2 Antibody (AF2084)(1)

Publication using AF2084

• Labbé, P;Martel, C;Shi, YF;Montezano, A;He, Y;Gillis, MA;Higgins, MÈ;Villeneuve, L;Touyz, R;Tardif, JC;Thorin-Trescases, N;Thorin, E; Knockdown of ANGPTL2 promotes left ventricular systolic dysfunction by upregulation of NOX4 in mice Frontiers in physiology 2024-02-15 [PMID: 38426206] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: ANGPTL2
• Uniprot:
  • Q9UKU9()