Aminopeptidase O/ONPEP Antibody [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

Aminopeptidase O/ONPEP Antibody [Alexa Fluor® 647] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Aminopeptidase O/ONPEP isoform 1
  Met1-Phe819
  Accession # Q8N6M6
• Specificity: Detects human Aminopeptidase O/ONPEP in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Aminopeptidase O/ONPEP Antibody [Alexa Fluor® 647]

• Aminopeptidase O
• AOPEP
• APO
• AP-OFLJ55832
• C90RF3
• C9orf3
• chromosome 9 open reading frame 3
• EC 3.4.11.-
• FLJ14675
• FLJ40923
• ONPEP

Background

Aminopeptidase O (AP-O; also C9orf3) is a 93 kDa (predicted), intracellular member of the M1 metalloprotease family of enzymes. It is expressed in heart, testis and pancreas where it apparently cleaves N terminal Arg and Asn residues. Human Aminopeptidase O is 819 amino acids in length. It contains one catalytic domain (aa 167‑589) with a Zn-binding site (His479-His483) and a C terminal ARM region (aa 706-819). At least three additional isoforms exist. One shows a deletion of aa 455‑553; a second has an 11 aa substitution for the C terminal 265 amino acids; and a third shows both a deletion of aa 455‑553 and an 11 aa substitution for the C-terminal 46 amino acids. Full length human Aminopeptidase O is 76% aa identical to mouse Aminopeptidase O.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.