AMIGO Antibody (S86-36) - BSA Free

Western Blot: AMIGO Antibody (S86-36) [NBP2-22413] - Western Blot analysis of Rat Brain Membrane showing detection of 60-80 kDa AMIGO-1 protein using Mouse Anti-AMIGO-1 Monoclonal Antibody, Clone S86-36 (NBP2-22413). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-AMIGO-1 Monoclonal Antibody (NBP2-22413) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 60-80 kDa.

Immunocytochemistry/Immunofluorescence: AMIGO Antibody (S86-36) [NBP2-22413] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-AMIGO-1 Monoclonal Antibody, Clone S86-36 (NBP2-22413). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-AMIGO-1 Monoclonal Antibody (NBP2-22413) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) AMIGO-1 Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence: AMIGO Antibody (S86-36) [NBP2-22413] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-AMIGO-1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X.

Immunocytochemistry/Immunofluorescence: AMIGO Antibody (S86-36) [NBP2-22413] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-AMIGO-1 Monoclonal Antibody, Clone S86-36 (NBP2-22413). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-AMIGO-1 Monoclonal Antibody (NBP2-22413) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) AMIGO-1 Antibody. (D) Composite.

Product Discontinued

AMIGO Antibody (S86-36) - BSA Free Summary

• Immunogen: Fusion protein amino acids 554-574 (cytoplasmic C-terminus) of human AMIGO-1
• Localization: Cell Membrane , Cell Projection , Axon
• Specificity: Detects approx 60-80kDa depending on maturity/glycosylation.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AMIGO1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
• Application Notes: Use in Flow reported in scientific literature (PMID:35273332) 1 ug/ml of AMIGO-1 Antibody was sufficient for detection of AMIGO-1 in 20 ug of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody..

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for AMIGO Antibody (S86-36) - BSA Free

• adhesion molecule with Ig-like domain 1
• ali2
• Alivin-2
• AMIGO
• AMIGO1
• AMIGO-1
• AMIGOamphoterin-induced protein 1
• amphoterin-induced gene and ORF
• KIAA1163

Background

Promotes growth and fasciculation of neurites from cultured hippocampal neurons. May be involved infasciculation as well as myelination of developing neural axons. May have a role in regeneration as well as neuralplasticity in the adult nervous system. May mediate homophilic as well as heterophilic cell-cell interaction andcontribute to signal transduction through its intracellular domain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AMIGO Antibody (NBP2-22413)(1)

Publication using NBP2-22413

• Goldhammer N, Kim J, Villadsen R et al. Myoepithelial progenitors as founder cells of hyperplastic human breast lesions upon PIK3CA transformation Communications biology 2022-03-10 [PMID: 35273332] (FLOW, Human)

Bioinformatics

• Gene Symbol: AMIGO1
• Uniprot:
  • Q86WK6()