ALS2CL Overexpression Lysate

Images

 
Western Blot: ALS2CL Overexpression Lysate (Adult Normal) [NBP2-04811] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ALS2CL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ALS2CL Overexpression Lysate Summary

Description

ALS2CL Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC213063

Accession#: NM_182775

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
ALS2CL

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
34.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for ALS2CL Overexpression Lysate

  • ALS2 C-terminal like
  • ALS2 C-terminal-like protein
  • DKFZp686I0110
  • DKFZp686P238
  • FLJ36525
  • MGC129698
  • RN49018

Background

ALS2CL acts as a guanine nucleotide exchange factor (GEF) for Rab5 GTPase. Regulates the ALS2-mediated endosomedynamics

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-14284
Species: Hu
Applications: ICC/IF, IHC,  IHC-P
NB120-13253
Species: Bv, Hu, Pm, Mu, Rt
Applications: ICC/IF, IHC-FrFl, IHC,  IHC-P, IP, WB
NBP3-41240
Species: Hu, Mu
Applications: WB
AF2780
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, WB
NBP1-58359
Species: Hu
Applications: IHC,  IHC-P, WB
NBP2-25160
Species: Bv, Ca, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, WB
236-EG
Species: Hu
Applications: BA
NBP1-88878
Species: Hu, Mu, Rt
Applications: IHC,  IHC-P, Simple Western, WB
NBP1-85032
Species: Hu
Applications: ICC/IF, IHC,  IHC-P
DAN00
Species: Hu
Applications: ELISA
AF640
Species: Mu
Applications: IHC, WB
NBP1-82145
Species: Hu
Applications: ICC/IF, IHC,  IHC-P
NBP1-52300
Species: Hu, Mu
Applications: ICC, ICC/IF, IHC,  IHC-P, WB
AF6260
Species: Hu
Applications: WB
NB110-68800
Species: Hu, Mu, Pm
Applications: ICC/IF, WB
AF4309
Species: Hu, Mu
Applications: ICC, IHC, WB
7570-GH
Species: Hu
Applications: EnzAct
NB600-607
Species: Hu, Rt
Applications: ELISA, IHC,  IHC-P, WB

Publications for ALS2CL Lysate (NBP2-04811) (0)

There are no publications for ALS2CL Lysate (NBP2-04811).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ALS2CL Lysate (NBP2-04811) (0)

There are no reviews for ALS2CL Lysate (NBP2-04811). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ALS2CL Lysate (NBP2-04811) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional ALS2CL Products

Blogs on ALS2CL

There are no specific blogs for ALS2CL, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ALS2CL Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol ALS2CL