AlphaB Crystallin/CRYAB Antibody [Biotin]

Product Discontinued

AlphaB Crystallin/CRYAB Antibody [Biotin] Summary

• Immunogen: A synthetic peptide made to a C-terminal region of the human Crystallin AB protein (within residues 100-175). [Swiss-Prot P02511]
• Predicted Species: Mouse (92%), Rat (100%), Porcine (100%), Bovine (100%), Rabbit (100%), Hamster (100%), Sheep (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRYAB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: This Crystallin AB antibody is useful for Western blot analysis, where a band is seen at ~20 kDa. There is also a band of greater intensity at ~40 kDa that may be an aggregated heteromeric complex. **CryAB aggregates with homologous proteins, including CryAA and the small heat shock protein HSP28, to form large heteromeric complexes. Use in ELISA, Flow Cytometry reported in scientific literature (PMID: 23586607).
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Reactivity Notes

Predicted to react with rat, sheep, rabbit, monkey, hamster, cow, and pig based on 100% sequence homology. Immunogen sequence has 92% homology to mouse. Mouse reactivity reported in scientific literature (PMID: 23586607).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for AlphaB Crystallin/CRYAB Antibody [Biotin]

• alpha B crystallin
• alpha(B)-crystallin
• AlphaB Crystallin
• alpha-crystallin B chain
• CRYA2
• CRYA2alpha crystallin B chain
• CRYAB
• crystallin, alpha B
• CTPP2
• Heat shock protein beta-5
• heat-shock 20 kD like-protein
• HSPB5
• Renal carcinoma antigen NY-REN-27
• Rosenthal fiber component

Background

Crystallin AB (CryAB) is a developmentally regulated small heat shock protein known to protect cells from stress damage. It may also play an important role in the Golgi reorganization during cell division. CryAB is known to be associated with a number of neurodegenerative pathologies such as Alzheimer disease amd multiple sclerosis. As well, it has been found to be expressed in breast tumors of patients with shorter survival, suggesting that it may be a useful marker in identifying aggressive breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AlphaB Crystallin/CRYAB Antibody (NB100-2520B)(1)

Publication using NB100-2520B

• Chauhan VS, Nelson DA, Marriott I, Bost KL. Alpha beta-crystallin expression and presentation following infection with murine gammaherpesvirus 68. Autoimmunit 2013-04-16 [PMID: 23586607] (ELISA, FACS, Mouse)
  
  Details:
  Using the Biotin conjugated version of NB100-2520, catalog number NB100-2520B.

Bioinformatics

• Gene Symbol: CRYAB