AlphaB Crystallin/CRYAB Antibody [Unconjugated]

Western blot shows lysates of mouse eye lens and human, mouse, and rat heart tissue. PVDF membrane was probed with 0.2 µg/mL Human/Mouse/Rat AlphaB Crystallin/CRYAB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4849) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human CRYAA and CRYAB (5 ng/lane) were included. A specific band for AlphaB Crystallin/CRYAB was detected at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western lane view shows lysates of human heart tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for AlphaB Crystallin/CRYAB at approximately 29 kDa (as indicated) using 2 µg/mL of Goat Anti-Human/Mouse/Rat AlphaB Crystallin/CRYAB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4849) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

AlphaB Crystallin/CRYAB Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human AlphaB Crystallin/CRYAB
  Met1-Lys175
  Accession # P02511
• Specificity: Detects human, mouse and rat AlphaB Crystallin/CRYAB in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human CRYAA is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CRYAB
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 2 ug/mL
  • Western Blot 0.2 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AlphaB Crystallin/CRYAB Antibody [Unconjugated]

• alpha B crystallin
• alpha(B)-crystallin
• AlphaB Crystallin
• alpha-crystallin B chain
• CRYA2
• CRYA2alpha crystallin B chain
• CRYAB
• crystallin, alpha B
• CTPP2
• Heat shock protein beta-5
• heat-shock 20 kD like-protein
• HSPB5
• Renal carcinoma antigen NY-REN-27
• Rosenthal fiber component

Background

AlphaB Crystallin (CRYAB, also known as Crystallin alpha -B chain and HspB5) is a 22‑23 kDa member of the HSP20 family of proteins. It has widespread expression in many tissues in addition to lens epithelium, where it noncovalently oligomerizes with CRY-alpha A to generate a transparent 350‑1000 kDa alpha -Crystallin protein complex. Human CRYAB is 175 amino acids (aa) in length. There is a alpha -Crystallin Hsp domain over aa 66‑149. Multiple posttranslational modifications may exist. The
N‑terminal Met and MetAspIleAlaIleHis sequence is occasionally cleaved. There is also phosphorylation at Ser45 and 59, potential O-GlcNAc modification at Thr158, 162 or 170, and acetylation at Lys92. There is an alternate start site at Met68 that may be accompanied by a 47 aa substitution for aa 109‑175. Full-length human CRYAB is 54% aa identical to CRY-AlphaA, and 98% aa identical to mouse CRYAB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CRYAB
• Uniprot:
  • P02511(Human)