AlphaB Crystallin/CRYAB Antibody (1B6.1-3G4) - BSA Free

Western Blot: AlphaB Crystallin/CRYAB Antibody (1B6.1-3G4) [NBP1-97494] - Crystallin AB Antibody (1B6.1-3G4) Lane 1: MW marker, Lane 2: alpha-Crystallin, Lane 3: alpha A Crystallin, Lane 4: alpha B Crystallin, Lane 5: alpha-Crystallin.

Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (1B6.1-3G4) [NBP1-97494] - Crystallin AB Antibody (1B6.1-3G4) Human Heart tissue stained with alpha B-Crystallin, mAb (1B6.1-3G4) at 10ug/ml.

• Reactivity: Hu, Mu, Rt, Bv, Ch
• Applications: WB, ICC/IF, IHC
• Clone: 1B6.1-3G4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

AlphaB Crystallin/CRYAB Antibody (1B6.1-3G4) - BSA Free Summary

• Description: Novus Biologicals Mouse AlphaB Crystallin/CRYAB Antibody (1B6.1-3G4) - BSA Free (NBP1-97494) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-AlphaB Crystallin/CRYAB Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Bovine Crystallin AB.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CRYAB
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 10ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1:1000
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for AlphaB Crystallin/CRYAB Antibody (1B6.1-3G4) - BSA Free

• alpha B crystallin
• alpha(B)-crystallin
• AlphaB Crystallin
• alpha-crystallin B chain
• CRYA2
• CRYA2alpha crystallin B chain
• CRYAB
• crystallin, alpha B
• CTPP2
• Heat shock protein beta-5
• heat-shock 20 kD like-protein
• HSPB5
• Renal carcinoma antigen NY-REN-27
• Rosenthal fiber component

Background

Alpha-crystallins, which are part of the small Heat shock family members, are major water-soluble proteins present in the lens of the mammalian eye. Phosphorylation of serine residues which occurs during development and in response to stress, is intimately linked with its function. Chaperone activity requires, and is modulated by, oligomerization and is limited to binding unfolded intermediates to prevent irreversible aggregation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AlphaB Crystallin/CRYAB Antibody (NBP1-97494)(5)

Publications using NBP1-97494

• Molina-Jijon E, Aparicio-Trejo OE, Rodriguez-Munoz R et al. The nephroprotection exerted by curcumin in maleate-induced renal damage is associated with decreased mitochondrial fission and autophagy Biofactors 2016-07-13 [PMID: 27412471] (WB, IF/IHC, Human)
• T Pannabecker et al. Loop of Henle interaction with interstitial nodal spaces in the renalinner medulla. Am. J. Physiol. Renal Physiol. 295, F1744 . 2008-01-01 [PMID: 18842821] (IF/IHC, Rat)
• TW Mu et al. Chemical and biological approaches synergize to ameliorate protein-folding diseases. Cell 134, 769 . 2008-01-01 [PMID: 18775310] (WB, Human)
• N Rao et al. Elevated retina-specific expression of the small heat shock protein,aA-crystallin, is associated with photoreceptor protection in experimental uveitis. Invest. Ophthalmol. Vis. Sci. 49, 1161 . 2008-01-01 [PMID: 18326745] (WB, Mouse)
• VL Cryns et al. The small heat shock protein alpha B-crystallin negatively regulates cytochrome c- and caspase-8-dependent activation of caspase-3 by inhibiting its autoproteolytic maturation. J. Biol. Chem. 276, 16059. 2001-01-01 [PMID: 11274139] (WB, Mouse)

Bioinformatics

• Gene Symbol: CRYAB