AlphaA Crystallin/CRYAA Antibody [Alexa Fluor® 750]

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 750

AlphaA Crystallin/CRYAA Antibody [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human AlphaA Crystallin/CRYAA
  Met1-Ser173
  Accession # P02489
• Specificity: Detects mouse AlphaA Crystallin/CRYAA in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human CRYAB is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AlphaA Crystallin/CRYAA Antibody [Alexa Fluor® 750]

• AlphaA Crystallin
• CRYA1
• CRYA1crystallin, alpha-1
• CRYAA
• crystallin, alpha A
• Heat shock protein beta-4
• HSPB4
• HSPB4alpha-crystallin A chain
• human alphaA-crystallin (CRYA1), 10HspB4

Background

AlphaA Crystallin (CRYAA, also known as Crystallin alpha -A chain and HspB4) is a 21‑22 kDa member of the HSP20 family of proteins. It is found in lens epithelium where it noncovalently oligomerizes with CRY-alpha B to generate a transparent 350‑1000 kDa alpha -Crystallin protein complex. Human CRYAA is 173 amino acids (aa) in length. There is a alpha -Crystallin Hsp domain over aa 63‑145. Multiple posttranslational modifications may exist. The C-terminal Ser and the N-terminal MetAspVal is occasionally cleaved. There is also phosphorylation at Ser45 and 122, O-linked GlcNAc modification at Ser162, deamidation at Asn101, and acetylation at Lys70. Full‑length human CRYAA is 54% aa identical to CRY-AlphaB, and 95% aa identical to mouse CRYAA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.