AlphaA Crystallin/CRYAA Antibody (OTI3B12) - Azide and BSA Free

Images

 
Western Blot: AlphaA Crystallin/CRYAA Antibody (OTI3B12) - Azide and BSA Free [NBP2-70450] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAA.
Immunocytochemistry/ Immunofluorescence: AlphaA Crystallin/CRYAA Antibody (OTI3B12) - Azide and BSA Free [NBP2-70450] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CRYAA.
Immunohistochemistry: AlphaA Crystallin/CRYAA Antibody (OTI3B12) - Azide and BSA Free [NBP2-70450] - Analysis of Human prostate tissue.
Immunohistochemistry: AlphaA Crystallin/CRYAA Antibody (OTI3B12) - Azide and BSA Free [NBP2-70450] - Analysis of Adenocarcinoma of Human endometrium tissue.
Immunohistochemistry: AlphaA Crystallin/CRYAA Antibody (OTI3B12) - Azide and BSA Free [NBP2-70450] - Analysis of Adenocarcinoma of Human ovary tissue.
Immunohistochemistry: AlphaA Crystallin/CRYAA Antibody (OTI3B12) - Azide and BSA Free [NBP2-70450] - Analysis of Carcinoma of Human liver tissue.
Immunohistochemistry: AlphaA Crystallin/CRYAA Antibody (OTI3B12) - Azide and BSA Free [NBP2-70450] - Analysis of Carcinoma of Human thyroid tissue.
Immunohistochemistry: AlphaA Crystallin/CRYAA Antibody (OTI3B12) - Azide and BSA Free [NBP2-70450] - Analysis of Human breast tissue.
Immunohistochemistry: AlphaA Crystallin/CRYAA Antibody (OTI3B12) - Azide and BSA Free [NBP2-70450] - Analysis of Human pancreas tissue.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI3B12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

AlphaA Crystallin/CRYAA Antibody (OTI3B12) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human CRYAA(NP_000385) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CRYAA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Western Blot 1:2000
Theoretical MW
19.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for AlphaA Crystallin/CRYAA Antibody (OTI3B12) - Azide and BSA Free

  • AlphaA Crystallin
  • CRYA1
  • CRYA1crystallin, alpha-1
  • CRYAA
  • crystallin, alpha A
  • Heat shock protein beta-4
  • HSPB4
  • HSPB4alpha-crystallin A chain
  • human alphaA-crystallin (CRYA1), 10HspB4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CRYAA