Western Blot: alpha-Synuclein [Nitrate Tyr125, Nitrate Tyr133] Antibody (24.8) [NBP1-26380] - (A) Nitrated Synuclein-alpha protein and (B) Non-nitrated Synuclein-alpha protein.
Immunohistochemistry-Paraffin: alpha-Synuclein [Nitrate Tyr125, Nitrate Tyr133] Antibody (24.8) [NBP1-26380] - Analysis of FFPE human brain using alpha-Synuclein antibody (clone 24.8) at 1:200 on a Bond Rx autostainer ...read more
Flow Cytometry: alpha-Synuclein [Nitrate Tyr125, Nitrate Tyr133] Antibody (24.8) [NBP1-26380] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HeLa (B) cells using Synuclein-alpha [Nitrate Tyr125, ...read more
This alpha-Synuclein antibody is useful for for Flow Cytometry, Immunohistochemisty paraffin embedded sections and Western blot, where a band is seen at ~14 kDa. Immunohistochemistry-Frozen was reported in scientific literature. Use in Immunofluorescence was reported in the scientific literature (PMID: 22033456).
14 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
Protein G purified
Alternate Names for alpha-Synuclein [Nitrate Tyr125, Nitrate Tyr133] Antibody (24.8)
Lewy body) 4
non A-beta component of AD amyloid
Non-A beta component of AD amyloid
Non-A4 component of amyloid precursor
non-A4 component of amyloid
synuclein, alpha (non A4 component of amyloid precursor)
alpha-Synuclein (SNCA, NACP or PARK-1) is a neuronal protein normally localized to presynaptic terminals and has been involved in processes such as dopamine biosynthesis, synaptic plasticity, and vesicle dynamics. SNCA-VAMP2 direct binding promotes SNARE complex assembly through a nonclassical chaperone activity and regulation of SNARE complex's assembly/disassembly is essential for efficient neurotransmission. CSP-alpha, a presynaptic protein also promotes SNARE complex assembly via formation of chaperone complex with heat shock cognate 70 (Hsc70) and small glutamine-rich protein SGT (CSP-alpha-Hsc70-SGT complex). Interestingly, the CSP-alpha-knockout mice depicted rapidly progressive neurodegeneration followed by premature death which was counteracted by transgenic expression of SNCA. Overexpressed SNCA indirectly inhibited SNARE-mediated exocytosis by sequestering arachidonic acid, which upregulates syntaxin and enhances its engagement with SNARE complex. Tight regulation of subcellular level and distribution of SNCA is indispensable for the intrinsic functions of neuronal cells and genetic alterations of SNCA resulting in aberrant polymerization into fibrils are associated with several neurodegenerative diseases (synucleinopathies) including Parkinson disease type -1 (PARK1) - 4 (PARK4) as well as dementia Lewy body (DLB).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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