alpha-Synuclein [Nitrate Tyr125, Nitrate Tyr133] Antibody (24.8)

Images

 
Western Blot: alpha-Synuclein [Nitrate Tyr125, Nitrate Tyr133] Antibody (24.8) [NBP1-26380] - (A) Nitrated Synuclein-alpha protein and (B) Non-nitrated Synuclein-alpha protein.
Immunohistochemistry-Paraffin: alpha-Synuclein [Nitrate Tyr125, Nitrate Tyr133] Antibody (24.8) [NBP1-26380] - Analysis of FFPE human brain using alpha-Synuclein antibody (clone 24.8) at 1:200 on a Bond Rx autostainer ...read more
Flow Cytometry: alpha-Synuclein [Nitrate Tyr125, Nitrate Tyr133] Antibody (24.8) [NBP1-26380] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HeLa (B) cells using Synuclein-alpha [Nitrate Tyr125, ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
24.8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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alpha-Synuclein [Nitrate Tyr125, Nitrate Tyr133] Antibody (24.8) Summary

Immunogen
Nitrated human alpha-Synuclein [Uniprot: P37840]
Epitope
2 nitrated residues Y125, Y133
Modification
Nitrate Tyr125, Nitrate Tyr133
Localization
Cytoplasm. Membrane. Nucleus. Membrane-bound in dopaminergic neurons.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SNCA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1500
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200-1:500
Application Notes
This alpha-Synuclein antibody is useful for for Flow Cytometry, Immunohistochemisty paraffin embedded sections and Western blot, where a band is seen at ~14 kDa. Immunohistochemistry-Frozen was reported in scientific literature. Use in Immunofluorescence was reported in the scientific literature (PMID: 22033456).
Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Brain Whole Tissue Lysate (Adult Whole Alzheimer's)
Publications
Read Publications using
NBP1-26380 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for alpha-Synuclein [Nitrate Tyr125, Nitrate Tyr133] Antibody (24.8)

  • alpha-Synuclein
  • Lewy body) 4
  • MGC110988
  • NACP
  • non A-beta component of AD amyloid
  • Non-A beta component of AD amyloid
  • Non-A4 component of amyloid precursor
  • non-A4 component of amyloid
  • PARK1
  • PARK4
  • SNCA
  • synuclein, alpha (non A4 component of amyloid precursor)
  • Synuclein-alpha

Background

alpha-Synuclein (SNCA, NACP or PARK-1) is a neuronal protein normally localized to presynaptic terminals and has been involved in processes such as dopamine biosynthesis, synaptic plasticity, and vesicle dynamics. SNCA-VAMP2 direct binding promotes SNARE complex assembly through a nonclassical chaperone activity and regulation of SNARE complex's assembly/disassembly is essential for efficient neurotransmission. CSP-alpha, a presynaptic protein also promotes SNARE complex assembly via formation of chaperone complex with heat shock cognate 70 (Hsc70) and small glutamine-rich protein SGT (CSP-alpha-Hsc70-SGT complex). Interestingly, the CSP-alpha-knockout mice depicted rapidly progressive neurodegeneration followed by premature death which was counteracted by transgenic expression of SNCA. Overexpressed SNCA indirectly inhibited SNARE-mediated exocytosis by sequestering arachidonic acid, which upregulates syntaxin and enhances its engagement with SNARE complex. Tight regulation of subcellular level and distribution of SNCA is indispensable for the intrinsic functions of neuronal cells and genetic alterations of SNCA resulting in aberrant polymerization into fibrils are associated with several neurodegenerative diseases (synucleinopathies) including Parkinson disease type -1 (PARK1) - 4 (PARK4) as well as dementia Lewy body (DLB).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for alpha-Synuclein Antibody (NBP1-26380)(3)

Reviews for alpha-Synuclein Antibody (NBP1-26380) (0)

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Product General Protocols

View specific protocols for alpha-Synuclein Antibody (NBP1-26380): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for alpha-Synuclein Antibody (NBP1-26380). (Showing 1 - 1 of 1 FAQs).

  1. I'm looking for an alpha-Synuclein antibody with an epitope located in the first half (N-terminus) of the protein - preferably a monoclonal antibody. Can you help me with that?
    • Please take a look at NB110-57475. It has been validated for human, rat and mouse and the applications ICC and WB and the epitope it detects is in the N terminal.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 405 NBP1-26380V
FITC NBP1-26380F

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Mechanisms of neurodegeneration: Mitochondrial dysfunction and oxidative stress
By Michalina Hanzel, PhDIn this second installment of our three blog-posts series on major cellular mechanisms responsible for neurodegenerative disorders, we will explore the processes of mitochondrial dysfunction an...  Read full blog post.

Mechanisms of Neurodegeneration: Protein aggregation and failure of autophagy
By Michalina Hanzel, PhDIn a series of three blog posts I will briefly explore the major cellular mechanisms responsible for many neurodegenerative disorders. The first, and perhaps the most apparent, is the accumulat...  Read full blog post.

The role of Parkin and autophagy in retinal pigment epithelial cell (RPE) degradation
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Bioinformatics

Gene Symbol SNCA
Entrez
Uniprot