Human alpha-N-acetylglucosaminidase/NAGLU ELISA Kit (Colorimetric)

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ELISA: Human alpha-N-acetylglucosaminidase/NAGLU ELISA Kit (Colorimetric) [NBP2-81175] - Samples were spiked with high concentrations of Human alpha-N-acetylglucosaminidase/NAGLU and diluted with Reference Standard & ...read more
ELISA: Human alpha-N-acetylglucosaminidase/NAGLU ELISA Kit (Colorimetric) [NBP2-81175] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL

Order Details

Human alpha-N-acetylglucosaminidase/NAGLU ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human alpha-N-acetylglucosaminidase/NAGLU in samples. No significant cross-reactivity or interference between Human alpha-N-acetylglucosaminidase/NAGLU and analogues was observed.
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.47%
Intra-Assay
CV% < 5.13%
Spike Recovery
84-101%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
NAGLU

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human alpha-N-acetylglucosaminidase/NAGLU ELISA Kit (Colorimetric)

  • alphaNacetylglucosaminidase
  • alpha-N-acetylglucosaminidase
  • EC 3.2.1.50
  • MPS3B
  • MPS-IIIB
  • N-acetyl-alpha-glucosaminidase
  • N-acetylglucosaminidase, alpha
  • NAG
  • NAGalpha-N-acetylglucosaminidase
  • NAGLU
  • UFHSD
  • UFHSD1

Background

NAGLU encodes an enzyme that degrades heparan sulfate by hydrolysis of terminal N-acetyl-D-glucosamine residues in N-acetyl-alpha-D-glucosaminides. Defects in this gene are the cause of mucopolysaccharidosis type IIIB (MPS-IIIB), also known as Sanfilippo syndrome B. This disease is characterized by the lysosomal accumulation and urinary excretion of heparan sulfate. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol NAGLU