alpha-Defensin 1 Overexpression Lysate (Native)

Images

 
Western Blot: Defensin alpha 1 Overexpression Lysate (Adult Normal) [NBL1-09818] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Defensin alpha 1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

alpha-Defensin 1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC218421 and based on accession number NM_004084. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens defensin, alpha 1 (DEFA1), mRNA.
Gene
DEFA1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
10 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for alpha-Defensin 1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for alpha-Defensin 1 Overexpression Lysate (Native)

  • alpha 2
  • alphaDefensin 1
  • alpha-Defensin 1
  • DEF1
  • DEF1DEFA1B
  • DEFA1
  • DEFA2
  • defensin, alpha 1, myeloid-related sequence
  • defensin, alpha 1HP-1
  • HNP-1
  • HNP-1HP1
  • HP1
  • MRS
  • MRSMGC138393
  • myeloid-related sequence
  • neutrophil defensin 1

Background

Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. It differs from defensin, alpha 3 by only one amino acid. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for alpha-Defensin 1 Lysate (NBL1-09818) (0)

There are no publications for alpha-Defensin 1 Lysate (NBL1-09818).
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FAQs for alpha-Defensin 1 Lysate (NBL1-09818). (Showing 1 - 1 of 1 FAQ).

  1. The gel picture shown above is what %?
    • The image you see for Western blot was obtained by running a gradient gel, 4-12%, in MOPS buffer.

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Bioinformatics

Gene Symbol DEFA1
Entrez